肾癌p53蛋白表达及其点突变相关研究  

p53 protein expession and gene point mutation in renal carcinomas

作　　者：俞天麟[1] 牛天力[1] 钱云程[1] 

机构地区：[1]兰州军区总医院泌尿外科

出　　处：《中华泌尿外科杂志》1999年第1期11-13,共3页Chinese Journal of Urology

基　　金：甘肃省自然科学基金

摘　　要：目的探讨肾细胞癌发病机制中致癌基因突变规律。方法以ｐ５３蛋白表达及其点突变率为实验观测指标，对以往１１６例各类肾肿瘤以免疫组化ＡＢＣ法检测病理切片的ｐ５３表达。并以ＰＣＲＳＳＣＰ观测５９外显子点突变，并以１２例非瘤新鲜肾组织作对照。结果三类恶性肾细胞癌的ｐ５３阳性表达率介于４２％～５４％之间，且随癌细胞恶性度增加及分期增加而升高，而良性瘤及正常肾组织为阴性。点突变率介于２５％～７１％之间，与恶性度及分期相关。第９外显子点突变率明显高于其它区域，良性瘤及正常肾无点突变。结论肾癌发病机制与ｐ５３基因突变密切相关。ｐ５３蛋白阳性表达与其点突变相辅并存，协同一致。Objective To investigate the mechenism of oncogene mutation in the genesis of renal cell carcinoma and to make clear the expression of p53 protein and its gene point mutation in renal carcinoma. Methods Sections of 116 cases of renal tumor with different patterns were examined for the expression of p53 protein by immunohistochemic ABC method.PCR SSCP method was used to detect the point mutation 5～9 exon of p53 gene in 92 cases of renal carcinoma and in 12 non tumor fresh kidney tissues as controls. Results The positive expression ratio of three patterns of renal carcinomas was between 42% and 54%, but the benign tumors and normal kidney tissues were negative for p53.The ratio of point mutation was between 25% and 71%,and the ratio of point mutation of nineth exon was remarkbly higher than other regions.The point mutation could not be deteced in benign tumor and normal kidney tissue. Conclusions The genesis of renal carcinoma is closely associated with gene mutation of p53,and both the oncoprotein and the point mutation play some role in carcinoma genesis.The point mutation is related to the malignent degree and the clinical stage.

关 键 词：肾肿瘤 癌 P53蛋白 基因突变 

分 类 号：R737.110.2[医药卫生—肿瘤]