微波消融联合CpG ODN治疗小鼠移植性结肠癌及对免疫状态的影响  

Therapeutic and immunological effects of microwave ablation combined with CpG ODN on transplanted colon carcinoma in mice

作　　者：邹宁[1] 李许锋[1] 丁为民[1] 来庆华[1] 张积仁[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心,广东广州510282

出　　处：《南方医科大学学报》2010年第5期984-988,共5页Journal of Southern Medical University

基　　金：卫生部科技专项基金(W2009BX015)

摘　　要：目的研究微波消融联合CpGODN对小鼠移植性结肠癌的治疗作用及对免疫状态的影响。方法 Balb/c小鼠皮下接种结肠癌CT26细胞建立肿瘤模型,肿瘤分别给予瘤周注射PBS、微波消融、微波消融+瘤周注射CpGODN和瘤周注射CpGODN四种处理。定期测量肿瘤大小,利用流式细胞仪检测小鼠外周血中CD3+CD4+T细胞、CD3+CD8+细胞的比例;采用ELISA检测小鼠外周血中IL-2、IL-12和IFN-γ含量。给予微波消融组和微波消融联合CpGODN组治愈的小鼠再次接种CT26细胞,观察成瘤率。结果微波消融组和微波消融联合CpGODN组小鼠的肿瘤体积显著小于PBS组和CpGODN组。4组小鼠外周血中的CD3+T细胞、CD3+CD4+T细胞、CD3+CD8+T细胞比例无显著性差异,微波消融联合CpGODN组及微波消融组的CD3+CD4+T/CD3+CD8+T细胞比值分别为1.58±0.10和1.53±0.13,与PBS组和CpGODN组相比显著升高(P均<0.05)。微波消融联合CpGODN组小鼠外周血中IL-2浓度为(64.6±7.4)pg/ml,IL-12浓度为(314.1±26.9)pg/ml,IFN-γ浓度为(61.9±7.3)pg/ml,显著高于其他3组(P均<0.05)。肿瘤再次攻击实验中微波消融联合CpGODN组成瘤率为25.0%,显著低于微波消融组的75.0%(P<0.05)。结论 CpGODN增强了微波消融治疗小鼠的免疫功能,减少了肿瘤再次攻击的成瘤率。Objective To investigate the therapeutic and immunological effects of microwave ablation （MA） combined with CpG ODN in mice bearing transplanted colon carcinoma. Methods A mouse model bearing colon carcinoma was established by subcutaneously inoculating CT26 cells into the right flank of Balb/c mice. The tumor-bearing mice were randomized into control group with PBS injection in the peritumoral area, MA group, MA combinated with CpG ODN group, and CpG ODN group with CpG ODN injection in the peritumoral area. The tumor volume changes were observed, and serum CD3＋CD4＋ and CD3＋CD8＋ T lmyphocytes were analyzed by flow cytometry after the treatments. Serum levels of interleukin （IL）-2, IL-12 and interferon-γ （IFN-γ） were detected by ELISA. The mice in the MA group and the combined treatment group showing tumor regression were rechallenged with CT26 cells. Results No significant difference was found in the number of serum CD3＋, CD3＋CD4＋, or CD3＋CD8＋ T lymphocytes between the 4 groups. The ratio of CD3＋CD4＋/CD3＋CD8＋ T lymphocytes in the combined treatment group and MA group were 1.58±0.10 and 1.53±0.13, respectively, significantly higher than that in PBS group and CpG ODN group （P0.05）. The serum concentration of IL-2, IL-12 and IFN-γ in the combined treatment group were 64.6±7.4 pg/ml, 314.1±26.9 pg/ml and 61.9±7.3 pg/ml, respectively, significantly higher than those in the other 3 groups （P0.05）. The tumor formation rate in the combined treatment group was significantly lower than that in MA group （25.0% vs 75.0%, P0.05）. Conclusion CpG ODN can enhance the immunity and decrease the tumor formation rate following a rechallenge with CT26 cells in mice treated with MA.

关 键 词：微波消融 CPG ODN 结肠肿瘤 免疫治疗 白细胞介素-2 白细胞介素-12 干扰素-γ 

分 类 号：R735.35[医药卫生—肿瘤]