实验性咬合紊乱对大鼠髁突软骨中PTHrP及其受体PTH1R表达的影响  

Effect of Experimentally Created Disordered Occlusion on the Expression of PTHrP and its Receptor PTH1R in Mandibular Condylar Cartilage of Rats

作　　者：付玉婷[1] 熊利峰[2] 于世宾[1] 张婧[1] 花翠[1] 蔡淑静[1] 王美青[1] 

机构地区：[1]第四军医大学口腔医学院解剖生理学教研室,陕西西安710032 [2]解放军477医院口腔科

出　　处：《口腔医学研究》2010年第3期305-308,共4页Journal of Oral Science Research

基　　金：国家自然科学基金资助(编号:30772429)

摘　　要：目的:探讨实验性咬合紊乱致大鼠髁突软骨异常改建过程中PTHrP及其受体PTH1R的表达变化情况。方法:在8周龄SD大鼠第二、三磨牙之间填塞正畸用橡皮筋推第三磨牙向远中移动,8周后取双侧颞下颌关节,苏木精-伊红染色观察组织形态变化,免疫组化及RT-PCR方法检测PTHrP及其受体PTH1R表达情况。结果:实验组髁突软骨后部明显增厚(P=0.032),且出现典型退行性变,软骨中PTHrP(P=0.034)及PTH1R(P=0.031)的mRNA表达明显低于空白对照组。结论:PTHrP及其受体PTH1R参与实验性咬合紊乱所致髁突软骨异常改建。Objective：To study the effect of experimentally created disordered occlusion（ECDO）on the expression of PTHrP and its receptor（PTH1R）in mandibular condylar cartilage of Sprague Dawley（SD）rats.Methods：In experiment group,a rubber band was inserted between the second molar and third molar on the left side of upper dentition and on the right side of lower dentition of Sprague Dawley（SD）rats aiming to move the third molars distally.Animals were sacrificed 8 weeks later.HE staining were carried out for studying the morphological changes of the condylar cartilage.Immunohistochemical staining and RT-PCR semi-quantitateive analysis were performed to detect the expression of PTHrP and PTH1R in the condylar cartilage.Results：In experimental group,the thickness of the condylar cartilage in the posterior region increased compared to the control group（P=0.032）.Furthermore,degenerative changes were found in the posterior part of the condyles in experimental group and the mRNA expression of PTHrP（P=0.034）and PTH1R（P=0.031）decreased.Conclusion：PTHrP and PTH1R play important roles in the pathological remodeling procedure in rat condylar cartilage induced by ECDO.

关 键 词：咬合 颞下颌关节 软骨 PTHRP PTH1R 

分 类 号：R782.6[医药卫生—口腔医学]