产低温脂肪酶菌株Psychrobacter sp.7342的筛选及粗酶性质研究  

Cold-adapted Lipase Produced by Psychrobacter sp.7342:Isolation and Enzyme Characterization

作　　者：陈锋菊[1] 谭树华[1] 黄凤玲[1] 贺玲[1] 

机构地区：[1]湖南科技大学生命科学学院,中国湖南湘潭411201

出　　处：《生命科学研究》2010年第3期199-202,共4页Life Science Research

基　　金：湖南省教育厅基金资助项目(B30939)

摘　　要：从南北极环境土样中筛选到1株产脂肪酶细菌7342,16SrDNA序列分析表明该菌株属于Psychrobacter sp..p-NPP法研究显示,菌株7342所产粗酶液的最适温度为30℃、最适pH值为8.0,对热较稳定;Co2+和Cs+对粗酶液有激活作用,而Na+、Sr2+等7种金属离子对其均有不同程度的抑制作用;粗酶液能在高浓度的SDS、Tween20等变性剂中表现出较好的稳定性.A strain 7342,which produces cold-adapted lipase,was isolated from the South Pole and North Pole environmental soil samples.16S rDNA sequence analysis showed it belonged to genus Psychrobacter.p-NPP research showed the optimal temperature of the Psychrobacter sp.7342 was 30 ℃,the optimal pH was 8.0,and it could resist to high temperature.Co2＋ and Cs＋ could activate the crude enzyme,and the seven metal ions as Na＋、Sr2＋ were able to inhibit enzyme activity.The crude enzyme had good stability in high concentrations of SDS,Tween 20 and other denaturants.

关 键 词：低温脂肪酶 筛选 酶学性质 

分 类 号：Q55[生物学—生物化学]