大孔树脂分离纯化脐橙皮中橙皮苷的工艺研究  

Studies on the isolation and purification of hesperidin from navel orange peel by macroporous resin

作　　者：杨佳[1] 张国文[1] 汪佳蓉[1] 冯利辉[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《食品科技》2010年第6期210-213,共4页Food Science and Technology

基　　金：科技部国家重点实验室项目(SKLF-MB-200807);江西省自然科学基金项目(2007GZH1924);江西省教育厅科技计划项目(GJJ08025)

摘　　要：以橙皮苷含量为指标,研究大孔树脂纯化赣南脐橙皮中橙皮苷的工艺条件,比较了D101、8、NⅡ和NKA-9 4种不同极性的大孔树脂对橙皮苷的静态吸附和解吸性能。结果发现,D101树脂纯化橙皮苷效果最好,其纯化最佳工艺是:上样浓度0.2 mg/mL,pH5,上样流速1 mL/min,洗脱液乙醇浓度60%(v/v),解吸流速0.5 mL/min,洗脱剂用量约4 BV。对经D101大孔树脂纯化后的橙皮黄酮进行高效液相色谱(HPLC)分析发现,橙皮苷含量达到91.41%,富集倍数为1.49倍,回收率为70.27%。D101大孔树脂可较好的分离纯化脐橙皮中的橙皮苷。A method of isolation and purification of hesperidin from navel orange peel in the south of Jiangxi province by macroporous resin was studied with hesperidin as the index.By comparing static adsorption and desorption properties of the four macroporous resins including D101,AB-8,NKA-Ⅱand NKA-9,the result indicated that the D101 type macroporous resin is the best for separating and purifying hesperidin from navel orange peel.The optimum conditions are： injecting concentration 0.2 mg/mL,pH5,injecting velocity 1.0 mL/min,60% alcohol as desorption solvent,desorption velocity of flow 0.5 mL/min and elution volume 4 BV.The analytical result show that the content of hesperidin after purification with D101 type resin is 91.41% by high performance liquid chromatography（HPLC）.The enrichment factor of hesperidin is 1.49 with recovery rate of 70.27%.Thus,it is feasible to separate and purify hesperidin by D101 type macroporous resin.

关 键 词：橙皮苷 大孔树脂 分离纯化 脐橙皮 

分 类 号：TS207.3[轻工技术与工程—食品科学]