高效液相色谱法检测茎椰菜中虫酰肼残留量  

Determination of tebufenozide residue in broccoli by high performance liquid chromatography

作　　者：石春红[1] 施燕萍[1] 许庆炎[1] 孙金才[1] 

机构地区：[1]海通食品集团股份有限公司,国家蔬菜加工技术研发分中心,宁波315300

出　　处：《食品科技》2010年第6期301-303,306,共4页Food Science and Technology

摘　　要：建立了分析茎椰菜虫酰肼残留量的高效液相色谱法。样品经丙酮提取,正己烷反相萃取,硅胶柱、胺基柱净化,以甲醇-水(75∶25,v/v)为流动相,用XDB-C18柱和紫外检测器对样品进行分离和测定。实验结果表明,虫酰肼添加浓度为0.05、0.2、1 mg/kg时,茎椰菜中虫酰肼的平均加标回收率为86.0%～93.4%,变异系数为1.56%～4.88%;在样品中虫酰肼的最低检出限为0.01mg/kg。该方法灵敏度高、准确性好,可用于茎椰菜中虫酰肼残留量的分析。An analytical method for determination of tebufenozide in broccoli by high performance liquid chromatography（HPLC） has been developed.Samples were extracted by acetone,then reversed-phase ex-tracted by n-hexane,and finally purified by LC-Si SPE and LC-NH2 SPE.Separation and quantification was achieved by using a XDB-C18 column and an ultraviolet（UV） detector.And a binary mobile phase of methanol-water（75∶25,v/v） was used.The average recoveries of tebufenozide in broccoli were 86.0%～93.4%,the coefficient of variation（CV,%） was 1.56～4.88%.The detection limit in samples was 0.01 mg/kg.In general,this method has turned out to be sensitive and accurate,suitable to analyze tebufenozide residue in broccoli.

关 键 词：虫酰肼 高效液相色谱法 残留 茎椰菜 

分 类 号：TS207.53[轻工技术与工程—食品科学]