PCR-SSCP法检测宫颈癌P^(53)基因突变  

Detection of P 53 gene mutation in cervical carcinoma by PCR-SSCP

作　　者：陈卫[1] 李文惠[1] 

机构地区：[1]兰州医学院生化教研室

出　　处：《肿瘤防治研究》1999年第1期16-17,共2页Cancer Research on Prevention and Treatment

基　　金：甘肃省自然科学基金

摘　　要：目的：探讨抑癌基因Ｐ５３突变与宫颈癌发生发展之间的关系，并为该病的临床检测提供一种分子生物学方法。方法：应用多聚酶链反应－单链构象多态性（ＰＣＲＳＳＣＰ）分析方法对宫颈癌及慢性宫颈炎组织中Ｐ５３基因５～６外显子的突变进行了检测。结果：３５例宫颈癌组织中有２例出现突变，突变率为５７１％；而作为对照的慢性宫颈炎组织中无一例出现突变。结论：宫颈癌的发生与抑癌基因Ｐ５３５～６外显子的突变有关；此方法可有效地检测抑癌基因Ｐ５３的突变。Objective To investigate the relationship between the progression and development in cervical carcinoma and the tumor suppressive gene P 53 and to supply a melecular biological method of clinical detection in this disease.Methods By using PCR-SSCP technology detected the mutations of exon 5～6 in P 53 gene in cervical carcinoma and chronic cervictitis tissues.Results The mutation was identified in 2 out of 35 cases of cervical carcinoma tissues.The mutation rate were 5 71%,The exon 5～6 in P 53 gene were not detected mutation in chronic cervisitis.Conclusion PCR-SSCP method is effective to detect gene mutation.P 53 gene mutation was relative in the development and progression of cervical carcinoma.

关 键 词：宫颈癌 P53基因 基因突变 PCR-SSCP 

分 类 号：R737.330.4[医药卫生—肿瘤]