衍生化技术在氨基酸分析中的应用进展  

Application progress of derivatization technology in amino acids analysis

作　　者：王秀中[1,2] 王清清[1] 宋海峰[1] 李卫平[2] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]安徽医科大学药理学教研室,合肥230032

出　　处：《药物分析杂志》2010年第6期1162-1166,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文结合不同仪器分析方法,包括反相高效液相色谱法(RP-HPLC)及液质联用法(HPLC-MS)、气相色谱法(GC)及气-质联用法(GC-MS)、毛细管电泳法(CE),对氨基酸分析方法中衍生化技术的最新应用进展进行综述。具体介绍了各氨基酸分析方法中所采用的衍生化方式和衍生化条件,讨论了各种衍生化方法的优缺点,并对氨基酸衍生化技术应用于和氨基酸相关的疾病诊断以及代谢组学的研究进行了展望。The review summarizes various derivatization reagents and corresponding derivatization processes of amino acid analysis in different method on the bases of different testing instruments and methodologies,including reverse phase high performance liquid chromatography（RP-HPLC）,high performance liquid chromatography-mass spectrometry（HPLC-MS）,gas chromatography（GC）,gas chromatography-mass spectrometry（GC-MS）and capillary electrophoresis（CE）.It discussed the advantage and disadvantage of all kinds of derivatiaztion methods mentioned and the prosmising prospect of the amino acid derivatiaztion techniques.The improvement of derivatization technology and sample pretreatment of amino acid in the fields of related disease diagnosis and metabolomics studies were prospected.

关 键 词：衍生化 氨基酸 仪器分析 

分 类 号：R917[医药卫生—药物分析学]