柱层析分离提纯麝香草酚的研究  

Study on separation and purification for thymol by column chromatography

作　　者：凌伟军[1] 余倩[1] 黄淋佳 余林[1] 麦裕良 林探厅[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]广东省石油化工研究院,广东广州510006

出　　处：《食品工业科技》2010年第7期242-244,247,共4页Science and Technology of Food Industry

基　　金：广东省化学工业公共实验室开放课题项目;广东省工业攻关项目(2008B060600004);广东工业大学博士启动基金(083057)

摘　　要：采用薄层层析和硅胶柱层析分离纯化了麝香草酚,并用气相色谱法测定纯化后的样品中麝香草酚的含量。薄层层析分离麝香草酚的适宜条件：点样量约2μL,在环己烷/无水乙醚（4/1,v/v）中展开,然后碘缸中显色15min,得到清晰的黄色斑点。硅胶柱层析分离烷基化产物中的麝香草酚的适宜条件：0.3mL的麝香草酚粗成品上10mm×400mm硅胶（60-100目）层析柱,流动相为环己烷/无水乙醚（4/1,v/v）,流速为0.4mL/min,分离出的麝香草酚纯度为99%。The thymol which was synthesized by alkylation of m-cresol and isopropanol were separated and purified by thin layer chromatography and silica gel column chromatography. The content of thymol in purified product was measured by gas phase chromatography. The optimum conditions for thin layer chromatography were confirmed：2μL of the sample solution were applied to silica gel G plates,while mixture of cyclohexane and anhydrous ether（4/1,v/v）was used as mobile phase. After detected in the Iodine-cylinder,there appeared three clear and concentrated spots. The optimum condition for silica gel column were confirmed：0.3mL of rude reaction product was applied to silica gel column（60~100mesh）while mixture of cyclohexane and anhydrous ether（4/1,v/v）was used as mobile phase. The flow rate was 0.4mL/min. The most content of separated thymol was 99%.

关 键 词：麝香草酚 薄层层析 柱层析 硅胶吸附剂 

分 类 号：TS201.2[轻工技术与工程—食品科学]