高效液相色谱法测定南瓜果肉中胡芦巴碱含量  

HPLC Determination of Trigonelline in Pumpkin Pulp

作　　者：徐雅琴[1] 王翀[1] 崔崇士[2] 

机构地区：[1]东北农业大学理学院,黑龙江哈尔滨150030 [2]东北农业大学园艺学院,黑龙江哈尔滨150030

出　　处：《食品科学》2010年第10期209-211,共3页Food Science

基　　金：黑龙江省自然科学基金资助重点项目(ZJN0606-01);黑龙江省教育厅科学技术研究项目(11521028)

摘　　要：以脱脂南瓜粉为原料,70%乙醇溶液为溶剂,微波辅助萃取样品中的胡芦巴碱,建立南瓜果肉中胡芦巴碱含量的高效液相色谱(HPLC)检测方法。实验过程中,采用的色谱柱为Agilent-ZORBXSB-C18(4.6mm×150mm,5μm)柱,流动相为0.40mmol/L磷酸溶液(pH3.2),流速为0.6mL/min,进样量为10μL,检测波长265nm,柱温为室温。研究结果表明,胡芦巴碱在5.6～134.4μg/mL范围内具有良好的线性关系,相关系数r=0.9999(n=5);平均回收率为97.09%,RSD为1.02%(n=5)。本法简便、准确、重复性好,可用于南瓜果肉中胡芦巴碱的含量测定。此外,采用此方法测定了13个品种南瓜果肉中胡芦巴碱的含量,结果表明胡芦巴碱含量为172.5～475.5μg/g。A method for the determination of trigonelline in pumpkin flesh was established using high performance liquid chromatography （HPLC）. Samples were defatted in a Soxhlet extractor with absolute ethyl ether as solvent and extracted with 70% aqueous ethanol with microwave assistance. An Agilent Zorbax SB-C18 column kept at ambient temperature was used for the chromatographic separation with 0.40 mmol/L aqueous solution of phosphoric acid （pH 3.2） as a mobile phase at a flow rate of 0.6 mL/min. The injection volume was 10 μL. The detection wavelength was set at 265 nm. Results showed that the developed standard curve of trigonelline exhibited good linearity over the concentration of 5.6 to 134.4 μg/mL, with a correlation coefficient of 0.9999 （n = 5）. The average recovery for trigonelline in a total of 6 real samples spiked at 3 levels repeated twice each in 5 replicates was 97.09%, with a relative standard deviation of 1.02%. This method proved to be simple, accurate and of good repeatability so as to be most suitable for the determination of trigonelline in pumpkin pulp. Thirteen cultivars of pumpkin pulp were determined by the method to have very different trigonelline contents ranging between 172.5μg/g and 475.5 μg/g.

关 键 词：南瓜 胡芦巴碱 高效液相色谱法(HPLC) 

分 类 号：TS218.1[轻工技术与工程—粮食、油脂及植物蛋白工程]