免疫磁珠捕获结合荧光PCR技术检测食品中黄曲霉菌的研究  

Immunomagnetic Capture-Fluorescent PCR (IMC-FPCR) for Rapid Detection of Aspergillus flavus in Foods

作　　者：苏惠玉[1] 谢荣珍[2] 牛建军[2] 

机构地区：[1]厦门医学高等专科学校,福建厦门361008 [2]厦门市疾病预防控制中心,福建厦门361021

出　　处：《食品科学》2010年第10期287-291,共5页Food Science

基　　金：厦门市科技计划项目(3502Z20064028)

摘　　要：目的:研究利用免疫磁珠对食品中黄曲霉菌进行捕获的技术,为检测食品中污染的黄曲霉菌提供新方法。方法:应用山羊抗黄曲霉菌血清的免疫磁珠捕获食品中的黄曲霉菌,不需要进行增菌培养,应用磁捕获-荧光聚合酶链式反应(IMC-FPCR)技术快速检测鉴定食品中的黄曲霉菌。结果:IMC-FPCR方法检测黄曲霉菌的最低检测值为2CFU/mL。应用IMC-FPCR对从市场上购买的12种食品进行检测,发现有两份样品含有产毒素相关基因(即为可能的致癌菌株),检出率为16.7%,其中花生中检出1株黄曲霉菌,面线中检出1株黄曲霉菌。结论:建立了黄曲霉菌的磁捕获-荧光聚合酶链式反应(IMC-FPCR)方法,该方法可用于食品中黄曲霉菌的快速检测及鉴定,实物检测结果提示市场上食品有被真菌污染。An immunomagnetic bead based technique combined with fluorescent PCR assay was developed for the detection of Aspergillus flavus in foods. Aspergillus flavus in foods could be captured with the immunomagnetic beads coated with goat antiserum against Aspergillus flavus without the requirement for fungal enrichment and rapidly detected by fluorescent PCR assay. The IMC-FPCR assay exhibited a limit of detection of 2 CFU/mL. Two of 12 commercial food samples were detected by the IMC-FPCR assay to contain toxin genes from carcinogenic fungal strains and the detection rate was 16.7% and one Aspergillus flavus strain was detected in peanut and Xiamen ready-to-eat noodles, respectively. This assay is most suitable for the rapid detection of Aspergillus flavus in foods.

关 键 词：黄曲霉菌 免疫磁珠 荧光PCR 快速检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]