高脂日粮改变小鼠股骨基因表达谱的聚类分析  

CLUSTERING ANALYSIS ON FEMORAL GENE EXPRESSION PROFILES IN MICE FED HIGH-FAT DIET

作　　者：肖瀛[1] 崔珏[1] 施用晖[2,1] 孙进[1] 乐国伟[2,1] 

机构地区：[1]江南大学食品学院,无锡214122 [2]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《营养学报》2010年第3期221-224,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30571347)

摘　　要：目的研究饲喂高脂日粮对小鼠股骨基因表达谱的影响。方法 4w龄C57BL/6雄性小鼠,体重13～14g,饲养正常日粮4d后,根据体重随机分为对照组(基础日粮)和高脂日粮组(19.5%猪油),每组8只小鼠,饲养12w后处死,迅速分离股骨,每组4份50mg股骨,提取RNA后等量合并,应用Affymetrix MOE430A小鼠基因表达芯片获得股骨基因表达谱变化的信息,通过DAVID在线分析工具进行聚类分析。结果长期饲喂高脂日粮导致C57BL/6小鼠股骨基因显著表达差异主要涉及如下功能:阳离子通道、信号转导和转入调控、骨矿化、磷代谢调控和胶原合成。结论长期摄入高脂日粮导致小鼠骨组织众多骨代谢相关基因表达改变,造成骨形成减少。Objective To investigate the changes in femoral gene expression profiles in C57BL/6 mice fed high-fat diet （HFD） via clustering analysis of DNA microarray.Method Sixteen male C57BL/6 mice （4w old） were randomly assigned to two groups,8 in each,after 4-d ordinary diet for adaptation.The control group was fed with an ordinary diet,and the HFD group with HFD（19.5% lard）.All mice were sacrificed at the end of 12w and the femoral gene expression was detected by oligonucleotide microarray analysis with Affymetrix Gene Chip Mouse U430A.DAVID,an online tool,was used for clustering analysis on femoral gene expression.Results Longtime administration of HFD caused femoral gene expressed differences related to cation ion channel,transcription regulation and signal transduction,bone mineralization,phosphate metabolic process regulation,and collagen synthesis.Conclusion Longtime intake of HFD will change the expression of numerous bone metabolism-related genes in bone of mice,and then inhibit bone formation.

关 键 词：基因表达谱 聚类分析 骨密度 高脂日粮 

分 类 号：R151[医药卫生—营养与食品卫生学]