两种共轭亚油酸对肥胖大鼠血清脂蛋白组成影响的比较研究  

THE COMPARATIVE STUDY ON THE EFFECT OF TWO KINDS OF CONJUGATED LINOLEIC ACID ISOMERS ON THE COMPOSITION OF SERUM LIPOPROTEIN IN FATTY RATS

作　　者：王玉明[1] 张蓓[1] 薛勇[1] 李兆杰[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《营养学报》2010年第3期230-233,238,共5页Acta Nutrimenta Sinica

基　　金：国家科技支撑计划(No.2008BAD94B01)

摘　　要：目的比较研究两种主要共轭亚油酸(conjugated linoleic acid,CLA)异构体对肥胖大鼠血脂不同粒径脂蛋白水平的影响。方法以OLETF(Otsuka Long-Evans Tokushima fatty rats)肥胖大鼠为实验动物,对照组喂食AIN76合成饲料,CLA组在饲料中分别添加1%(以质量计)的9c,11t-CLA(9ct组)和1%(以质量计)的10t,12c-CLA(10tc组),喂食2w。以试剂盒方法测定血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-c)浓度。采用HPLC方法按脂蛋白粒径大小,对血清脂蛋白进行细化分析,测定不同粒径中TC和TG浓度。结果 10t,12c-CLA使血清各脂蛋白中TG浓度均显著下降,总TG浓度降低了22.5%,血清小粒径LDL和大粒径HDL中TC浓度显著增加,总TC浓度增加了26.1%。而9c,11t-CLA使血清总TG增加了22.6%,对总TC浓度无显著影响。结论 10t,12c-CLA具有降低血清TG,升高血清HDL-c的效果,但其对动脉粥样硬化的影响仍有待深入研究。Objective To investigate the effects of the two major conjugated linoleic acid（CLA） isomers on serum lipoprotein composition in fatty rats.Method Eighteen male OLETF rats were randomly divided into three groups.The control group fed with AIN76 diets,CLA groups were fed with 1% 9c,11t-CLA （9ct group） or 1%10t,12c-CLA （10tc group） containted AIN76 diets.After two weeks,serum triglyceride （TG）,total cholesterol （TC）,and high density lipoprotein cholesterol （HDL-c） were determined by commercial kits.On the other hand,serum lipoprotein were separated into chylomicron（CM）,very low density lipoprotein（VLDL）,low density lipoprotein（LDL） and HDL by HPLC according to the different particle sizes,and the TC and TG levels were measured in each lipoprotein.Results 10t,12c-CLA feeding reduced the concentrations of rat serum TG significantly,and increased the concentration of serum TC （26.1%） by increasing TC levels of the small particle size LDL and the big particle size HDL.While 9c,11t-CLA feeding increased the serum TG by 22.6%,and had no effect on the serum TC.Conclusion 10t,12c-CLA can reduce the concentration of serum TG and increase the concentration of HDL-c,but the effect on the improvement of atherosclerosis still need further investigation.

关 键 词：共轭亚油酸 脂蛋白 高脂血症 密低密度脂蛋白 

分 类 号：R151[医药卫生—营养与食品卫生学]