响应面分析法优化海地瓜酶解工艺及其抗氧化活性的研究  

STUDY ON OPTIMIZATION OF ENZYMOLYSIS TECHNOLOGY OF ACAUDINA LEUCOPROCTA BY RESPONSE SURFACE METHOD AND THEIR ANTIOXIDANT ACTIVITIES

作　　者：侯付景[1] 金春华[1] 董明敏[1] 李妍妍[1] 邵亮亮[1] 苏秀榕[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,宁波315211

出　　处：《营养学报》2010年第3期276-280,共5页Acta Nutrimenta Sinica

基　　金：国家农业科技成果转化资金资助项目(No.2007GB2C220359);宁波市科技成果转化资金资助项目(No.2007C100055);宁波市科技局国际合作项目(No.2008C500265);浙江省重点社会发展项目

摘　　要：目的为了充分发挥海地瓜的生理功能,优化酶解工艺。方法本研究选用复合蛋白酶,以羟自由基清除率为标准,利用响应面分析法(RSM)优化水解条件。将最优条件下制得海地瓜水解液,igICR小鼠,42d后,测定小鼠血清和肝脏组织匀浆液中SOD,GSH-Px活力和MDA含量。结果研究结果显示:最佳的水解条件为:复合蛋白酶加酶量2.02%;酶解温度51.18℃;酶解时间127.81min,此时羟自由基清除率为73.10%。海地瓜水解液使小鼠血清和肝脏组织中的SOD,GSH-Px活力明显提高,MDA含量显著降低。结论该条件下海地瓜酶水解液具有良好的抗氧化作用。Objective To optimize the enzymolysis condition for Acaudina leucoprocta to research lencoprocta the antioxidant activity of its hyoholysate in vivo.Method Response surface methodology （RSM） was employed to optimize the hydrolysis conditions with protamex.The ICR mice were given ig the hydrolysate of Acaudina lencoprocta obtained under optimal conditions.The activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） and the content of MDA in blood serum and liver in mice were analyzed.Results and Conclusion The best hydrolysis conditions were：the amount of enzyme was 2.02%;enzymolysis temperature was 51.18 ℃;enzymolysis time was 127.81min,hydroxyl radical scavenging rate was 73.10%.The activities of SOD,GSH-Px in hydrolysate were significantly enhanced and the content of MDA was significantly lower,which showed that this hydrolysate had remarkable antioxidation activity.So the enzymolysis technology for Acaudina leucoprocta was optimal.

关 键 词：海地瓜 响应面 酶解 抗氧化 

分 类 号：TQ461[化学工程—制药化工]