华佗健聪汤对β淀粉样蛋白损伤的神经元存活率和细胞形态的影响  

Effect of Huatuo Jiancong decoction on the survival rate of neuron and cellular shapes damaged by Aβ

作　　者：袁晓琳[1] 江励华[1] 

机构地区：[1]南京中医药大学基础医学院,210046

出　　处：《中国临床研究》2010年第6期449-451,F0002,共4页Chinese Journal of Clinical Research

摘　　要：目的研究华佗健聪汤对β淀粉样蛋白(Aβ)损伤的神经元的存活率和细胞形态的影响。方法取原代培养的神经细胞,随机分为正常组、中药组、模型组、阳性对照组。正常组神经细胞以无血清Neuralbasal培养基培养,不予以任何干预措施;模型组以浓度为10μmol/L的Aβ对神经细胞予以干预;中药组:将含华佗健聪汤血清加入无血清Neuralbasal培养基中培养神经细胞24h后,弃培养基,加入Neuralbasal培养基及10μmol/LAβ继续培养;阳性对照组:将中药组的含药血清换成未灌中药的正常大鼠血清及给予终浓度为20μmol/L人参皂苷Rg1,余均与中药组相同。培养24h后,观察并拍摄细胞形态,MTT实验评价细胞活力。结果华佗健聪汤组与模型组相比,细胞形态结构基本完好,OD值增高(P<0.05)。中药组细胞成活率为82.64%,显著高于模型组细胞成活率(64.76%)(P<0.05)。结论华佗健聪汤对Aβ所致的大鼠神经元损伤有较好的保护作用。Objective To observe the effect of Huatuo Jiancong decoction on the survival rate of neuron and cellular shapes damaged by amyloid-beta protein（Aβ）.Methods The nerve cells with primary culture were randomly divided into normal group,Chinese medicine group （Huatuo Jiancong decoction group）,model group and positive control group.The nerve cells in normal group were cultured with the serum-free Neuralbasal culture medium and were not given any interventions.The nerve cells in model group underwent the intervention of 10 μmol/L Aβ.The nerve cells in Huatuo Jiancong decoction group were cultured with the Neruralbasal culture medium joined by serum with Huatuo Jiancong decoction for 24 hours,then the culture medium was poured off,and the nerve cells were continuously cultured with the Neuralbasal medium and 10 μmol/L Aβ.The serum with Huatuo Jiancong decoction in Huatuo Jiancong decoction group were subsititued by the normal serum of rats which were not administered Huatuo Jiancong decoction,and 20 μmol/L Rg1 was given in positive control group,and the others in positive control group were same as those in Huatuo Jiancong decoction group.24 hours after nerve cells cultivation,the cell shapes were observed and the cell activity was determined by MTT assay.Results Compared with model group,the cells shapes were basically intact,and the OD values increased in Huatuo Jiancong decoction group.The survival rate of nerve cells in Huatuo Jiancong decoction group was significantly higher than that in model group（82.64% vs 64.76%,P0.05）.Conclusions Huatuo Jiancong decoction has protective effect on neuron injury of rats.

关 键 词：阿尔茨海默病 华佗健聪汤 Β淀粉样蛋白 神经元存活率 细胞形态 

分 类 号：R749.16[医药卫生—神经病学与精神病学]