姜黄素诱导人胃癌SGC-7901细胞凋亡  

Curcumin induces apoptosis of human gastric carcinoma SGC-7901 cells

作　　者：李玉芳[1] 仲宁[1] 沈文香[1] 王金志[2] 

机构地区：[1]昆山市第一人民医院肿瘤科,215300 [2]苏州大学医学院分子生物学教研室

出　　处：《江苏医药》2010年第11期1278-1280,F0002,共4页Jiangsu Medical Journal

摘　　要：目的探讨姜黄素诱导人胃癌SGC-7901细胞凋亡的作用。方法采用噻唑蓝(MTT)比色法检测人胃癌SGC-7901细胞生长活力;Hoechst33258核染色观察细胞形态学变化;流式细胞术检测细胞周期及细胞凋亡率;比色法检测Caspase-3、8、9活性。结果姜黄素(5.0、10.0、20.0、40.0、80.0μmol/L)明显抑制SGC-7901细胞生长并呈剂量依赖关系,48h的IC50值为(23.65±3.15)μmol/L,细胞核经Hoechst33258染色出现浓染致密的固缩形态和颗粒状荧光。与对照组相比,姜黄素处理后凋亡细胞比例增加;姜黄素处理SGC-7901细胞48h后Caspase-3、8、9的活性明显增强。结论姜黄素可通过激活Caspase级联活化而抑制人胃癌SGC-7901细胞生长并诱导其凋亡。Objective To study the effect of curcumin on apoptosis of human gastric carcinoma SGC-7901 cells.Methods Cell viability was measured by MTT assay in human gastric carcinoma SGC-7901 cells.Morphological changes of cell nuclei were observed by Hoechst 33258 staining.Flow cytometry was used to measure the cell apoptosis.The relative activities of Caspase 3,8 and 9 were tested by colorimetry.Results Curcumin（5.0,10.0,20.0,40.0 and 80.0μmol/L）induced growth inhibition of SGC-7901 cells in a dose-dependent manner.The IC50 value at 48h was（23.65±3.15）μmol/L.Cell nucleus concentrated and appeared granular fluorescence by Hoechst 33258 staining.The percentage of apoptotic cells increased in curcumin treated groups compared with that in the control group.After SGC-7901 cells were treated with curcumin for 48h,the activities of Caspase 3,8 and 9 were significantly increased.Conclusion Curcumin induces apoptosis of human gastric carcinoma SGC-7901 cells by activating Caspases.

关 键 词：姜黄素 胃癌 细胞凋亡 CASPASE 

分 类 号：R735[医药卫生—肿瘤]