高效液相色谱法测定鸡蛋清和蛋黄中磺胺类药物残留  

Determination of Residual Sulfonamides in Egg White and Yolk by HPLC

作　　者：吴翠琴[1] 陈迪云[1] 黄燕屏[1] 谢棣君[1] 邓红梅[1] 

机构地区：[1]广州大学环境科学与工程学院,广东广州510006

出　　处：《环境监测管理与技术》2010年第3期43-45,64,共4页The Administration and Technique of Environmental Monitoring

基　　金：广东省自然科学基金资助项目(8451009101001037);国家自然科学基金资助项目(40803026);广州大学新苗计划项目;广州大学引进人才科研启动项目

摘　　要：采用高效液相色谱法测定鸡蛋清和蛋黄中磺胺嘧啶、磺胺吡啶、磺胺二甲基嘧啶、磺胺甲氧哒嗪、磺胺甲恶唑、磺胺间甲氧嘧啶、磺胺喹恶啉等7种磺胺类药物残留,优化了色谱条件和样品预处理方法。7种磺胺类药物在0.050 mg/L^10.0 mg/L范围内线性良好,检出限和定量限分别低于0.02 mg/kg和0.04 mg/kg。在0.250 mg/kg和0.500 mg/kg两个添加水平,蛋清中的回收率为81.0%~100%,RSD为0.8%~4.8%,蛋黄中的回收率为72.2%~94.1%,RSD为1.1%~6.9%。A high-performance liquid chromatography（HPLC） method for the multi-residues simultaneous determination of sulfadiazine,sulfapyridine,sulfamethazine,sulfamethoxypyridazine,sulfamethoxazole,sulfamonomethoxine,sulfaquinoxaline in egg white and yolk was described.The test parameters of HPLC and sample pretreatment were optimized.The results indicated that a good linearity was in range from 0.050 mg/L to 10.0 mg/L.The detection limits and quantity limits were lower than 0.02 mg/kg and 0.04 mg/kg.At levels of 0.250 mg/kg and 0.500 mg/kg,the spiked recoveries of egg white were between 81.0% and 100%,RSDs were between 0.8% and 4.8%;and the spiked recoveries of yolk were between 72.2% and 94.1%,RSDs were between 1.1% and 6.9%.

关 键 词：磺胺类药物 高效液相色谱法 鸡蛋清 鸡蛋黄 

分 类 号：O657.72[理学—分析化学]