宇佐美曲霉L336酸性蛋白酶的动力学性质  

STUDIES ON THE KINETIC PROPERTY OF ACID PROTEINASE FROM ASPERGILLUS USAMII MUTANT L336

作　　者：翁醒华[1] 徐晨明[1] 李永泉[1] 胡华萃 周红军[1] 贺筱蓉[1] 

机构地区：[1]杭州大学生命科学学院,杭州310028

出　　处：《菌物系统》1999年第1期61-66,共6页Mycosystema

基　　金：浙江省教育委员会科研基金!9415138

摘　　要：L336酸性蛋白酶的分子量为54000，等电点为3．8±0．2。在pH2．5，40℃条件下，酶对酪蛋白、牛血清白蛋白和牛血红蛋白的Km值分别为0．263g／L、0．278g／L和0．415g／L，Vmax分别为2075、1550和2793μgTyr／min、mL，酪蛋白为最适底物。在pH2．0～5．0、40℃以下时酶的稳定性最好。此外，还研究了金属离子对酶活性的影响。The L336 acid proteinase from Aspergillus usamii was shown to have a molecular weight of 54000 and an isoelectric point of 3.8± 0.2. The Km Values of the enzyme at 40℃ and pH2.5 for casein、bovine serum albumin and bovin haemoglobine were 0.263g / L. 0.278g / L and 0.415g / L reapectively, and the Vmax values under the same conditions were 2075. 1550 and 2793μg Tyr / min、mL. The optimum substrate for acid proteinase was casein. The enzyme was stable at temperature up to 40℃ and pH2.5~5.0. In addition, the effect of different metal ions on the activities of enzyme was also studied.

关 键 词：霉菌 酸性蛋白酶 动力学性质 

分 类 号：Q936[生物学—微生物学] Q949.32