应用原位杂交及RAPD技术标记抗黄矮病小麦一中间偃麦草染色体异附加系  

In situ Hybridization and RAPD Analyses of Disomic Thinopyrum intermedium Addition Lines in Wheat with Barley Yellow Dwarf Virus Resistance

作　　者：徐琼芳[1] 马有志[1] 辛志勇[1] 陈孝 林志珊[1] 

机构地区：[1]中国农业科学院作物所农业部作物遗传育种重点开放实验室,北京100081

出　　处：《Acta Genetica Sinica》1999年第1期49-53,共5页

基　　金：国家"863"计划";国家自然科学基金

摘　　要：以生物素（Ｂｉｏｔｉｎ－１６－ｄＵＴＰ）标记中间偃麦草基因组 ＤＮＡ为探针，与抗黄矮病小麦－中间偃麦草染色体异附加系Ｚ６进行原位杂交，鉴定出附加的１对中间偃麦草染色体。对异附加系 Ｚ６和 Ｌ１及它们的小麦亲本进行了 ＲＡＰＤ分析，从 １２０个随机引物中，筛选出 ２个引物可以扩增出附加染色体的特异ＤＮＡ片段，可作为鉴定寻人小麦的中间偃麦草染色质的分子标记。The genomic DNA of Thinopyron intermedium labelled with biotin-16-dUTP was hybridized with the chromosomal DNA of Wheat-Th. intermedium addition Line Z, (2n = 44), which had a good resistance to barley yellow dwarf vims (BYDV). As a result a pair of Th. intermedium chromosomes added in wheat background was identified by the in situ hybridization. Furtherly, Z6, L, (a wheat-Th. intermedium addition line with BYDV resistance) and their parents were analyzed by RAPD analysis, and 2 primers by which the specific DNA of the alien chromosomes in Z6 or L1 could be amplified were screened from 120 ones. The special DNA amplified by PCR could be used as the molecular marker to detect Th. intermedium chromatin in wheat background.

关 键 词：异附加系 原位杂交 小麦 抗黄矮病 中间偃麦草 

分 类 号：S512.101[农业科学—作物学] Q942[生物学—植物学]