丙酮酸肌酸对大鼠胴体组成及机体氨基酸谱的影响  

Effect of Creatine Pyruvate on Carcass Characteristics and Amino Acid Compositions in Rats

作　　者：孔一力[1] 刘仪[1] 张源淑[1] 苏小倩[1] 马海田[1] 邹思湘[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《食品科学》2010年第11期214-220,共7页Food Science

基　　金：国家自然科学基金项目(30600439)

摘　　要：以大鼠为实验对象,灌胃丙酮酸肌酸(CrPyr),通过观测其对机体胴体组成及其血清与组织中氨基酸谱的影响,探讨丙酮酸肌酸对机体中营养物质的代谢流向及其胴体品质的影响。实验选用40只雄性清洁级SD大鼠,随机分为对照组(C)、低剂量组(L)、中剂量组(M)和高剂量组(H)4个处理组,每个处理组按体质量分别灌胃0、750、1500、3000mg/(kgbw·d)的CrPyr。实验为期7周,第49天宰杀大鼠,借助反相高效液相色谱(RP-HPLC)方法测定血清、组织中氨基酸含量的变化。结果表明:与对照组相比,在不影响饲料转化率的情况下,灌胃CrPyr可显著(P<0.05)地提高其腿肌的相对质量;灌胃CrPyr可显著(P<0.05)或极显著(P<0.01)升高大鼠血清和骨骼肌中主要生糖氨基酸的含量,但对肝脏组织的氨基酸谱则没有明显影响。提示,灌胃CrPyr可能通过升高生糖氨基酸的含量,从而促进大鼠体蛋白的合成。In order to investigate the effect of creatine pyruvate（CrPyr） on metabolic direction of nutritive substances,rats were administered CrPyr to explore carcass characteristics and amino acid compositions in serum and tissues.Forty healthy male SD rats were randomly divided into four groups,which were administered CrPyr at the dosages of 0,750,1500 mg/（kg bw·d） and 3000 mg/（kg bw·d）,respectively.On the 49th day of the experiments,all rats were scarified after 12 h fasting.The content of amino acids in serum and tissues were determined by reversed-phase high performance liquid chromatography（RP-HPLC）.Results showed that relative weight of leg muscle in CrPyr-treated groups was significantly increased（P 0.05） compared with the control group.Similarly,CrPyr-treated groups exhibited a significant increase in the content of glucogenic amino acid in serum and muscle.However,no obvious difference in amino acid composition in liver was observed.These investigations suggested that CrPyr could improve protein synthesis by elevating the concentration of glucogenic amino acid in serum and muscle of rats.

关 键 词：大鼠 丙酮酸肌酸 氨基酸谱 

分 类 号：Q555.7[生物学—生物化学]