松香基功能高分子稀土离子配合物固定化糖化酶  

Immobilization of Glucoamylase by Rosin Based Functional Polymer-Rare Earth Ion Complexes

作　　者：袁霖[1] 卢建芳[2] 雷福厚 段文贵[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530006 [2]广西民族大学化学与生态工程学院,广西南宁530006

出　　处：《化学与生物工程》2010年第6期62-65,共4页Chemistry & Bioengineering

基　　金：广西科学研究与技术开发基金资助项目(桂科攻08395003-9)

摘　　要：分别以聚马来松香己二醇酯(PMHE)、马来松香丙烯酸乙二醇酯聚合物(PMAGE)和马来松香丙烯酸乙二醇酯-丙烯酸共聚物[Poly(MAGE-AA)]为载体,以Y3+为桥键配离子固定化糖化酶,并测定了固定化糖化酶的性能。结果表明,Poly(MAGE-AA)Y En固定化酶的性能较好,最适温度为50℃,比游离酶高出10℃;最适pH值为5.01;重复使用5次后,活性为1888.00 U.g-1,说明固定化糖化酶的稳定性增强。Immobilized glucoamylase were prepared by using polymer of maleated rosin acid-hexandiol ester（PMHE）,copolymer of maleated rosin acid-acrylic acid-glycol ester-acrylic acid [Poly（MAGE-AA）] and polymer of maleated rosin acid-acrylic acid-glycol ester（PMAGE） as carriers and the method of rare earth ion coordination.The performances of the immobilized enzymes were determined.The result showed that the highest activity was observed for the Poly（MAGE-AA）YEn.The optimal temperature and pH value was 50℃ and 5.01,respectively.The immobilization enzyme activity was 1888.00 U·g-1 after used for 5 cycles.It proved that the stability of immobilized glucoamylase was increased.

关 键 词：松香基功能高分子 糖化酶 固定化酶 稀土离子 

分 类 号：Q814[生物学—生物工程]