层柱粘土载体的制备、表征及固定β-D-葡萄糖醛酸苷酶的研究  

Characterization of PILC Carrier and Immobilization of β-D-glucuronidase

作　　者：党艳艳[1] 李春[1] 张根林[1] 冯世江[1] 

机构地区：[1]新疆兵团化工绿色过程重点实验室/石河子大学食品学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2010年第3期357-359,共3页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(20776017);北京市自然科学基金项目(5072028)

摘　　要：为提高甘草及膨润土的附加值及酶的固定化寻找新载体提供一种新方法,采用新疆夏子街膨润土作基质粘土,以十六烷基三甲基溴化铵（CTAB）为模板剂,正硅酸四乙酯（TEOS）为硅源制备层柱粘土,采用吸附法固定化β-D-葡萄糖醛酸苷酶,并对该固定化酶载体进行表征和酶活测定。结果表明：层柱粘土的孔径分布在2-50nm,属于中孔吸附剂;XRD分析大量的硅溶胶进入粘土层间,其d001值为0.982nm;FT-IR分析显示酶与层柱粘土未结合的羟基发生了氢键缔合而被固定,最高固定化酶活可达1460U/g载体。By using cetyl trimethyl ammonium bromide as the template agent,the four ethyl silicate silicon source for preparation of pillared interlayered clay（PILC） from bentonite was studied.Absorption method was used to immobilize β-D-glucuronidase using PILC as support.The character of PILC carrier and activity of immobilized β-D-glucuronidase were investigated.The results showed that the distribution of pore size of pillared clay was between 2~50nm;XRD analysis of the d001 value was 0.982 nm;FT-IR analysis showed that the enzyme was immobilized on the PILC by hydrogen bonds,the highest immobilized enzyme activity was up to 1460 U/g carrier.

关 键 词：膨润土 层柱粘土 固定化 β-D-葡萄糖醛酸苷酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]