嗜酸乳杆菌对致泻性大肠埃希氏菌上皮细胞粘附和侵袭的抑制作用  

Inhibition of adherence and invasiveness of diarrheogenic E. coli to Hep 2 cells by Lactobacillus acidophilus DOM La

作　　者：傅晓丽[1] 徐建国[1] 高守一[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所,卫生部医学分子细菌学重点实验室

出　　处：《中华微生物学和免疫学杂志》1999年第2期97-101,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金;杰出青年基金

摘　　要：目的研究嗜酸乳杆菌ＤＯＭＬａ菌株是否对致泻性大肠埃希菌对上皮细胞粘附和侵袭能力具有抑制作用。方法使用了Ｈｅｐ－２细胞粘附实验和Ｈｅｐ－２细胞侵袭实验。结果发现嗜酸乳杆菌ＤＯＭＬａ菌株对Ｈｅｐ－２细胞粘附平均达２２２．４８±３０．７个细菌／细胞。ｔｈｙＡ基因突变菌株ＤＯＭＬａＦ８４对Ｈｅｐ－２细胞的粘附为６１．９６±１０．２７个细菌／细胞。将嗜酸乳杆菌ＤＯＭＬａ菌株与肠产志贺氏样毒素且具有侵袭力的大肠埃希氏菌（ＥＳＩＥＣ）Ｆ１７１菌株共同孵育后，可将后者的粘附和侵袭菌数分别由２７３．４±２４．７个细菌／细胞和１３．４±４．９个细菌／细胞，降低到６５．３±１３．１个细菌／细胞和６．８２±１．６个细菌／细胞，差异有显著性（Ｐ＜０．０１）。将ＤＯＭＬａ菌株与肠侵袭性大肠埃希氏菌（ＥＩＥＣ）８４０１菌株共同孵育后，可将后者对Ｈｅｐ－２细胞的粘附和侵袭数分别由２６９．２±２６．５个细菌／细胞和３４．０±５．３个细菌／细胞降到７０．７±１４．０个细菌／细胞和６．８２±２．９个细菌／细胞，差异有显著性（Ｐ＜０．０１）。结论嗜酸乳杆菌ＤＯＭＬａ菌株对ＥＳＩＥＣＦ１７１菌株和ＥＩＥＣ８４０１菌株的Ｈｅｐ－２细胞的粘附和侵袭能力?Objective To select a L. acidophilus strain which has potential to be used as vector for vaccine development of enteropathogens. Methods Hep 2 cell culture on cover slips were incubated with the bacteria to be tested for 3h at 37℃. After incubation the cover slips were fixed with formalia and stained with Giemsa stain for “Single Bacteria culture” and with Grams stain for “Duel Bacteria culture”. After that the specimen were examed microscopically. Fifty adhered cells were counted for “Bacteria Adherence Test”. For “Bacteria Invasion Test” the co culture (Hep 2 cells and bacteria) had to be cultivated for further 1h after the previous 3h incubation. There after 50 invaded cells were counted. Results Hep 2 cell adherence assay data showed that L. acidophilus DOM La had intensive adhesive ability to Hep 2 cell line. The mean number of adherence of it was 222.48±30.7 organisms/ Hep 2 cell. The thyA mutant derivative strain of it was isolated, named as DOM LaF84, which retained the activity to adherence. The Hep 2 cell adherence and invasiveness of entero SLTs producing and invasive E. coli F171 were reduced by L. acidophilus DOM La from 273.4 ±24.7 organisms/cells and 13.4±4.9 organisms/ cell to 65.3±13.1 organisms/cell and 6.82± 1.6 organisms/Hep 2 cell respectively. The Hep 2 cell adherence and invasiveness of enteroinvasive E. coli 8401 were also reduced by L. acidophilus DOM La from 269.2±26.5 organisms/cell and 34.0±5.3 organisms/cell to 70.7±14 0 organisms/cell and 6.82±2.9 organisms/cell. Conclusions Based on these results, we concluded that the L. acidophilus DOM La strain has unspecific inhibition effects on the invasiveness and adherence ability of diarrheogenic E. coli , which could be used as a basic feature for development of lactic bacterial vaccine vector system.

关 键 词：嗜酸乳杆菌 细菌粘附 大肠杆菌 感染性腹泻 

分 类 号：R363.22[医药卫生—病理学]