木聚糖酶高产菌株的筛选及产酶条件  

Screening of a high xylanase producing strain and the study on the conditions for xylanase production

作　　者：沈进军[1,2,3] 王建军 朱逸涵[3] 张顺萍[3,5] 黄志伟[1,2] 

机构地区：[1]农业部海峡两岸农业技术合作中心,福建福州350002 [2]福建农林大学农产品品质研究所,福建福州350002 [3]福建农林大学生命科学学院,福建福州350002 [4]山东寿光第一中学,山东寿光262700 [5]福建农林大学菌物研究中心,福建福州350002

出　　处：《亚热带农业研究》2010年第2期115-120,共6页Subtropical Agriculture Research

基　　金：福建省科技创新平台建设计划项目(2007S1001);福建省科技重大专项专题项目(2008NZ0001-4)

摘　　要：从本实验室保存的46株纤维素分解菌中筛选出1株可高效降解半纤维素的木聚糖酶高产菌株APS35,其酶活力高达31.801IU.g-1,比APS02(对照)高4.91倍。产酶条件优化结果表明,以水稻秸秆为底物,APS35产木聚糖酶的最适条件:培养温度28℃,培养时间3-4d,稻草粉与麸皮比4∶1,接种量10%,氮源为酵母膏(总氮量0.4%),pH为4.0,吐温80浓度0.4%。在此条件下的木聚糖酶活力为32.024IU.g-1,与优化前无显著差异。A high xylanase producing strain of ＂APS 35＂ that can efficiently degrade hemicellulose was screened from 46 strains stored in our lab.The xylanase activity was up to 31.801 IU·g-1 which was 4.91 times higher than ＂APS 02＂（control）.The results of optimization of fermentation conditions showed that the optimum conditions that ＂APS 35＂ produced xylanase with rice straw as the substrate were culture temperature 28 ℃,inocubation time 3-4 days,straw powder and wheat bran ratio 4∶1,inoculation ratio 10%,yeast extract as nitrogen source（the amount of total nitrogen 0.4%）,pH 4.0,Tween-80 concentration 0.4%.Under these conditions,the xylanase activity was 32.024 IU·g-1,no significant difference from prior to optimization.

关 键 词：木聚糖酶 筛选 产酶条件 

分 类 号：Q939.99[生物学—微生物学]