预处理对脑微血管内皮细胞紧密连接的影响  

Effects of preconditioning on tight junctions of brain microvascular endothelial cells

作　　者：安平[1] 赵晓春[2] 薛一雪[1] 

机构地区：[1]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001 [2]中国医科大学附属盛京医院麻醉科,辽宁沈阳110004

出　　处：《中国现代医学杂志》2010年第11期1643-1646,1651,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30800451;30872656;30973079);辽宁省自然科学基金(No:20052102;20082102);辽宁省高等学校科研项目计划(No:2008850)

摘　　要：目的采用氧糖剥夺预处理模拟脑缺血预处理,探讨预处理对大鼠脑微血管内皮细胞(BMECs)紧密连接的影响。方法体外培养新生大鼠BMECs,给予细胞氧糖剥夺2.5h,再给氧糖3.0h,模拟在体缺血再灌注损伤,以0.5h氧糖剥夺作为预处理。观察细胞超微结构的改变,应用荧光检测细胞间紧密连接相关蛋白ZO-1和细胞骨架蛋白F-actin的位置变化。结果氧糖剥夺可破坏细胞紧密连接等超微结构,使BMECsZO-1和F-actin的膜定位发生改变。预处理可对内皮细胞起到明显的保护作用,稳定ZO-1和F-actin的膜定位。结论预处理可抑制BMECs紧密连接及相关蛋白的改变,对血脑屏障起保护作用。[Objective]To study the effects of preconditioning on tight junctions of brain microvascular endothelial cells, using oxygen glucose deprivation （OGD） to mimic ischemia in vivo. [Methods]Primary culturing the rat brain microvascular endothelial cells, were given 2.5 h OGD and 3 h re-oxygenation/glucose to mimic ischemiareperfusion in vivo with 0.5 h OGD as preconditioning. The changes of cell ultrastructure were tested, and the expression of tight junction proteins ZO-1 and F-actin was assessed by immunofluorescence and fluorescence-labeled assays. [Results]OGD could destroy the cell tight junction ultrastructure, and change the membrane location of ZO-1 and F-actin. OGD preconditioning could significantly alleviate the injury of brain microvascular endothelial cells, and lighten the changes of tight junction proteins ZO-1 and F-actin location. [Conclusion]OGD preconditioning could inhibit the changes of brain microvascular endothelial cells tight junctions and provide protective effects on blood brain barrier.

关 键 词：预处理 氧糖剥夺 紧密连接 ZO-1 F-ACTIN 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]