噬氨副球菌HPD-2固体发酵条件优化及其对PAHs污染土壤的修复效果  

Optimal Conditions of Solid State Fermentation on Paracoccus aminovorans HPD-2 and Its Remediation Potential in PAH-contaminated Soil

作　　者：刘增俊[1,2] 滕应[1] 骆永明[1,2] 赵静[1] 李振高[1] 

机构地区：[1]中国科学院土壤环境与污染修复重点实验室(南京土壤研究所),南京210008 [2]中国科学院研究生院,北京100049

出　　处：《土壤》2010年第3期404-409,共6页Soils

基　　金：中国科学院知识创新工程项目(KZCX2-YW-404;KSCX2-YM-GO53;CXTD-Z2005-4);国家"863"计划项目(2007AA061101;2004AA649210)资助

摘　　要：选用有机肥A、有机肥B和紫花苜蓿粉3种有机物料作为菌剂载体,探讨了固体发酵过程中物料量、接种量、固水比和发酵时间对噬氨副球菌HPD-2生长的影响,以及固体发酵菌剂对PAHs污染土壤的修复效果。结果表明,3种发酵载体中,以有机肥A的效果最好。噬氨副球菌HPD-2以有机肥A为载体的固体发酵最佳条件为物料量20g、接种量5%、固水比1:1、发酵时间144h。菌剂施入土壤28天后,土壤中PAHs总含量由初始的9.96mg/kg下降到7.64mg/kg,其去除率达22.8%。不同环数PAHs的去除率高低顺序为3环>5环>6环和4环,分别下降了35.1%、27.0%、20.7%、20.4%。该菌剂对PAHs污染土壤有一定的修复效果。The optimal conditions of solid state fermentation of Paracoccus aminovorans HPD-2 and the remediation effect of microbial agents on PAH-contaminated soil were studied. As for the solid state fermentation, we selected three kinds of organic materials included organic manure A, organic manure B and alfalfa, and discussed the effects of the amount of solid matrix, inoculum size, moisture content, incubation time and its remediation potential in PAH-contaminated soil. The results showed the best organic material was organic manure A and the optimal conditions for the bacteria growth in the solid state fermentation included 20 g solid matrix and 5% inoculum size, and 1：1 moisture content （w/v） with 144 h of incubation period. After 28 days, bioaugmented microcosms of microbial agents showed a 22.8% decrease in total PAHs, with the concentration declined from 9.96 mg/kg to 7.64 mg/kg. The degradation percentage of 3-ring, 5-ring, 4-ring and 6-ring PAH were 35.1%, 27.0%, 20.7%, 20.4%. respectively. This microbial agent had some extent remediation potential in PAH-contaminated soil.

关 键 词：多环芳烃 有机物料 噬氨副球菌HPD-2 固体发酵 生物修复 

分 类 号：X172[环境科学与工程—环境科学]