克鲁维酵母菌产胆盐水解酶发酵条件的优化研究  

Optimization of fermentation conditions of Kluyveromyces marxianus bile salt hydrolase

作　　者：刘慧[1] 董瑞婷[1] 潘昌莉[1] 高秀芝[1] 张红星[1] 

机构地区：[1]北京农学院食品科学学院,北京102206

出　　处：《中国酿造》2010年第7期44-46,共3页China Brewing

基　　金：北京市自然科学基金资助项目(No.5092008);北京市重点建设学科资助项目(PXM2009-014207-078172)

摘　　要：利用从藏灵菇中筛选的产胆盐水解酶(bile salt hydrolase,BSH)的马克斯克鲁维酵母菌K1(Kluyveromyces marxianus K1)研究提高酶活力的环境因素。针对主要影响胆盐水解酶合成的4个因素,采用4因素3水平L9(34)正交试验确定了高产胆盐水解酶优化发酵条件:发酵温度为32℃,发酵时间为24h,培养基的初始pH值为6.0,接种量为2%;在优化发酵条件下,K1菌株产BSH活力比优化前提高了52.4%,为开发研制降胆固醇功能食品及微生态制剂提供了试验基础。Effect of environmental factors on activity of bile salt hydrolase produced b y Kluyveromyces marxianus K1, which was isolated from Tibetan Kefir, was studied. The optimum fermentation conditions for high enzyme production were determined with orthogonal experiments L9（3^4） as follows： fermentation under 32% for 24 h, using medium with starting pH at 6.0 and inoculnm by 2%. Under the optimum conditions, activity of bile salt hydrolase was 52.4% higher than before.

关 键 词：马克斯克鲁维酵母菌K1 胆盐水解酶 优化条件 

分 类 号：Q93-335[生物学—微生物学]