产凝乳酶黑曲霉JG的微波诱变育种研究  

Screening of Aspergillus niger JG strain with high chymosin activity by microwave induced mutation

作　　者：邵淑娟[1,2] 李铁柱[2] 李倬林[2] 杨贞耐[1,2] 

机构地区：[1]吉林大学军需科技学院,吉林长春130062 [2]吉林省农业科学院农产品加工研究中心,吉林长春130033

出　　处：《中国酿造》2010年第7期47-50,共4页China Brewing

基　　金：国家高技术研究发展计划(863计划)(2006AA10Z306);农业部现代农业产业技术体系建设专项资金资助项目(nycytx-0502)

摘　　要：采用微波辐照方法对产凝乳酶的黑曲霉JG进行诱变处理,在酪蛋白培养基上以凝乳圈直径为指标进行初筛,在基础固态发酵培养基上进行复筛,选育出具有遗传稳定性的突变株WB6-3、WB2-5,凝乳活力分别比出发菌株提高了35%和14%。The starting strain Aspergillus niger JG producing chymosin was mutated by microwave irradiation. Effect of mutation was determined by preliminary screening based on size of milk-clotting zone and secondary screening based on solid fermentation medium. The mutant strains WB6-3 and WB2-5 with good genetic stability and high chymosin activity were obtained. Comparing with the starting strain, activity chymosin produced by WB6-3 and WB2-5 was increased by 35% and 14%, respectively.

关 键 词：凝乳酶 微波诱变 菌种改良 

分 类 号：Q93-331[生物学—微生物学]