复合酶法提取广佛手多糖的工艺研究  

Extraction of Polysaccharides from Bergamot by Compound Enzymolysis Method

作　　者：章斌[1] 李远志[2] 陈宇 郭奕纯 

机构地区：[1]韩山师范学院生物系,广东潮州521041 [2]华南农业大学食品学院,广东广州510640 [3]广东济公保健食品有限公司,广东潮安515638

出　　处：《安徽农业科学》2010年第15期7833-7835,7873,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省教育部产学研结合项目(2007A090302002)

摘　　要：[目的]探讨复合酶法对广佛手多糖提取率的影响。[方法]以广佛手为原料,用复合酶法提取多糖。通过单因素和L16(45)正交试验对佛手多糖的提取工艺进行优化。[结果]复合酶法提取佛手多糖的最佳工艺条件为:酶用量1.2%、酶解时间150min、料液比1∶40、酶解温度50℃,此条件下的佛手多糖得率为4.13%。[结论]复合酶法能显著地提高多糖的提取率。[Objective] To study the effects of compound enzymolysis method on bergamot polysaccharides and provide a theoretical basis for its application.[Method] The bergamot taken from Guangdong Province was used as experimental material,and the polysaccharides from it were extracted by compound enzymolysis method.The optimum extraction conditions were determined by single factor and L16（4^5）orthogonal experimental designs.[Result] The optimum conditions were as follows：compound enzyme amount 1.2%,enzymatic extraction time 150 min,solid-liquid ratio 1∶40 and enzymolysis temperature 50 ℃.Moreover,the extraction yield of polysaccharides is 4.13% under those conditions.[Conclusion] The yield of bergamot polysaccharides can be improved by compound enzymolysis method.

关 键 词：复合酶 佛手 多糖 

分 类 号：R284.2[医药卫生—中药学]