绿色木霉产纤维素酶菌种的优化及酶学性质研究  

Screening of Cellulase-producing Trichoderma viride Strains and Its Zymology Property

作　　者：秦涛[1] 李忠玲[1] 张强[1] 马齐[1] 

机构地区：[1]陕西省科学院酶工程研究所,陕西临潼710600

出　　处：《贵州农业科学》2010年第6期110-111,120,共3页Guizhou Agricultural Sciences

基　　金：国家星火计划项目"新型复合酶及微生物饲料添加剂的产业化开发"(2007EA850003)

摘　　要：为了研究纤维素酶的酶学性质,以产纤维素酶的绿色木霉为出发菌株,采用微波诱变处理,对其发酵生产的纤维素酶酶学性质进行研究。结果表明,绿色木霉经微波诱变与原有菌种相比产酶活力提高30%以上;该酶分别在37℃和pH 5.0时,酶活性最高;在羧甲基纤维素底物保护下,酶的热稳定性大幅度提高;金属离子Ba2+、K+对酶有激活作用,Cu2+、Ca2+、Fe2+、Mn2+、Zn2+、Fe3+对酶有抑制作用。The cellulase-producing Trichoderma viride stains were treated with microwave to study zymology property of cellulase.The results showed that the activity of cellulase-producing Trichoderma viride stain induced by microwave was 30% higher than that of origin Trichoderma viride stain,the cellulase activity was the highest at 37℃ and pH 5.0,the thermal stability of cellulase was greatly increased under protection of CMC,Ba2＋ and K＋ had activation effect on cellulase activity but Cu2＋,Ca2＋,Fe2＋,Mn2＋,Zn2＋ and Fe3＋ had inhibition effect on cellulase activity.

关 键 词：纤维素酶 微波诱变 酶学性质 纤维素酶活力 

分 类 号：Q55[生物学—生物化学]