火焰原子吸收法间接测定食物中植酸的研究  

Studies on the Determination of Phytic Acid in Foods by Flame Atomic Absorption Spectroscopy

作　　者：吕杰 肖志芳[1,2] 李耀根[1,2] 

机构地区：[1]广州市天河区卫生防疫站 [2]华西医科大学

出　　处：《中国卫生检验杂志》1999年第1期13-16,共4页Chinese Journal of Health Laboratory Technology

基　　金：CMB基金

摘　　要：本文建立了火焰原子吸收法间接测定食物中植酸含量的方法，对测试条件进行了系统的探讨。该方法的线性范围为０～２０μｇ植酸／ｍｌ，相关系数为０．９９９６，检出限为０．１４４μｇ植酸／ｍｌ，相对标准偏差为１．１％～６．６％，１５种样品的加标回收率为９６．１％～１０６．１％，该法与消化测磷法的测定结果比较，差异无显著性。该法灵敏度高，结果准确可靠，操作简便再稀释成浓度为１０μｇＰ／ｍｌ的应用液。１．２．８植酸标准溶液：精确称取０．６６４１ｇ植酸钾（Ｓｉｇｍａ公司，含量为９５．５５％），用水溶解并定容至１００ｍｌ，其浓度为６ｍｇ植酸／ｍｌ。用时再用水逐级稀释成浓度为３００μｇ植酸／ｍｌ的应用液。１．３分析方法１．３．１原子吸收分光光度计参数分析波长２４８．２ｎｍ，狭缝宽度０．２ｎｍ，灯电流５ｍＡ，空气流速１３．５Ｌ／ｍｉｎ，Ｃ２Ｈ２流速２．２Ｌ／ｍｉｎ，测量方式为面积积分。１．３．２标准曲线的绘制分别取植酸标准应用液（３００μｇ植酸／ｍｌ）０．００、２．００、４．００、６．００、８．００、１０．００ｍｌ于２５ｍｌ玻璃离心管中，各加入３．００ｍｌ铁标准溶液和２．００ｍｌ３．５ｍｏｌ／Ｌ氯化钠溶液，均加水至１５．００ｍｌ，?We have developed a method for determining phytic acid contents in foods by flame atomic absorption spectroscopy based in determining residual Fe 3+ in supernatant after precipitation of phytic acid with excessive Fe 3+ . The conditions for extraction of phytic acid in samples, the separation and purification of extract and the reaction between phytic acid and Fe 3+ , etc. were optimized. The linear range of the method, the correlation coefficient, the detection limit, relative standard deviation and recovery are 0～20μg phytic acid/ml, 0 9996,0 144μg phytic acid/ml, 1 1%～6 6% and 96 1%～106 1%, respectively, The reliability of the method was evaluated by comparison with the method based on phosphorus analysis(P>0 2). This method is sensitive, accurate, simple for analysis of Phytic acid in foods.

关 键 词：植酸 提取 分离 食物 火焰原子吸收 

分 类 号：R151.3[医药卫生—营养与食品卫生学]