高速逆流色谱分离纯化丰城鸡血藤中刺芒柄花素  

Separation of Formononetin from Millettia nitita var.hirsutissima Using High-Speed Counter-current Chromatography

作　　者：阮征[1,2] 吴昌强[1,2] 印遇龙[1,2,3] 邓泽元[1,2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学生命科学与食品工程学院,江西南昌330031 [3]中国科学院业热带农业生态研究所,湖南长沙410125

出　　处：《食品科学》2010年第12期93-96,共4页Food Science

基　　金：南昌大学“赣江学者奖励计划”项目;中国博士后科学基金一等资助项目(20080440166);江西省教育厅项目(GJJ09068)

摘　　要：目的:确定高速逆流色谱分离制备高纯度丰城鸡血藤黄酮类物质刺芒柄花素的条件。方法:利用高效液相色谱测定刺芒柄花素在两相溶剂体系中的分配系数K值,通过K值优化确定高速逆流色谱分离的两相溶剂体系,并测定刺芒柄花素的纯度。结果:用于高速逆流色谱分离的两相溶剂体系为:正己烷-乙酸乙酯-甲醇-水(4:5:4:5,V/V),体系的上相为固定相,下相为流动相。高速逆流色谱分离条件为:流速2mL/min,转速800r/min,检测波长260nm,温度26℃。从鸡血藤乙醚提取物中可一步纯化得到活性成分刺芒柄花素,得率为16.1%,高效液相色谱检测其纯度达96.3%。结论:该溶剂体系分离结果可靠,可作为高效快速分离纯化刺芒柄花素的制备分离方法。Purpose：To develop the experimental system for the separation of formononetin from Millettia nitita var.hirsutissima by high-speed counter-cunent chromatography（HSCCC）.Methods：The optimum solvent composition for HSCCC separation was determined based on partition coefficients and phase separation times for various fractions of the Millettia nitita var.hirsutissima.The purity of the formononetin fraction from HSCCC separation was measured by HPLC.Results：A mixture of n-hexane,ethylacetate,methanol and water（4：5：4：5,V/V） was the selected solvent system,the upper phase and the lower phase separated from which provided mobile phase and stationary phase,respectively.The HSCCC separation of formononetin was carried out under the following conditions：flow rate 2.0 mL/min,rotation speed 800 r/min and 26 ℃.The yield of formononetin purified from the ether extract from Millettia nitita var.hirsutissima was 16.1%,with 96.3% purity.Conclusion：The developed solvent system can provide a reliable separation of formononetin.The HSCCC method is applicable for the rapid and efficient separation of formononetin.

关 键 词：丰城鸡血藤 高速逆流色谱 刺芒柄花素 高效液相色谱 

分 类 号：TQ460.72[医药卫生—药物分析学]