FGF-2和TRX在非小细胞肺癌中的表达及临床病理学意义  

Clinicopathological significance of FGF-2 and TRX Expressions in non-small cell lung cancer

作　　者：刘玮[1] 邓征浩[1] 周建华[1] 李婷[1] 傅春燕[1] 高振芹[1] 陈晨[1] 

机构地区：[1]中南大学湘雅医学院病理学系,湖南长沙410078

出　　处：《中国现代医学杂志》2010年第10期1461-1465,共5页China Journal of Modern Medicine

基　　金：湖南省自然科学基金资助项目(NO:06JJ2098)

摘　　要：目的探讨成纤维细胞生长因子-2(FGF-2)和硫氧还蛋白(TRX)在非小细胞肺癌(NSCL)组织中的表达及其相关性。方法应用免疫组织化学SP法分析76例非小细胞肺癌组织、15例非肺癌肺组织中FGF-2和TRX蛋白的表达水平。采用Western印迹、RT-PCR方法检测外源性FGF-2对人肺腺癌细胞株A549中TRX蛋白及mRNA表达水平的影响。结果非小细胞肺癌组织中FGF-2和TRX阳性率分别为65.8%(50/76)和72.3%(55/76),显著高于非肺癌肺组织(P<0.01),FGF-2和TRX阳性表达与淋巴结转移、临床分期密切相关(P<0.01),与年龄、性别及病理分型无关(P>0.05)。两者表达在NSCLC中呈正相关(P<0.01)。FGF-2可以上调A549细胞中TRX蛋白和mRNA的表达。结论 FGF-2和TRX在非小细胞肺癌发生与发展中具有重要作用,FGF-2可能通过TRX促进肺癌的发生与发展。[Objective]To investigate the expression of fibroblast growth factor-2 （FGF-2） and Thioredoxin （TRX） in NSCLC （Non-small cell lung cancer） tissues and analyze the correlation between them. [Methods]The expression of FGF-2 and TRX were detected by immunohistochemistry in NSCLC tissues and non-lung carcinoma tissues. The expression of TRX protein and mRNA induced by FGF-2 in A549 cells were detected by Western blot and RT-PCR.[Results]The positive expressions of FGF-2 （65.8%） and TRX （72.3%） in NSCLC tissues were significantly higher than that in non-lung carcinoma tissues（13.3% and 26.7%, respectively） （P 0.01）. The expressions of FGF-2 and TRX were significantly related to TNM stages and lymph node metastasis （P 0.01）, but not to histological types, sex and age of NSCLC patients （P 0.05）. A positive correlation was found between the expressions of FGF-2 and TRX in NSCLC （P 0.01）. The expressions of TRX protein and mRNA were up-regulated in A549 cells when it was treated with FGF-2. [Conclusion]FGF-2 and TRX play important role in NSCLC, FGF-2 might promote the progression of NSCLC by up-regulating the expression of TRX.

关 键 词：FGF-2 TRX 非小细胞肺癌 A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]