反义肿瘤坏死因子α对小鼠眼内单疱病毒感染影响的实验研究  

Effects of TNF-α Antisense Oligonucleotides Treatment on Subconjunctival Herpes Simplex Virus(HSV)-1-induced Uveitis

作　　者：李劲 宋琳[2] 彭伟 朱格非 胡慧丽 聂爱芹 

机构地区：[1]暨南大学医学院附属第二医院深圳市人民医院眼科,深圳518020 [2]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《热带医学杂志》2010年第6期660-662,共3页Journal of Tropical Medicine

摘　　要：目的观察局部使用肿瘤坏死因子α反义寡核苷酸(TNF-α-ASON)对小鼠眼内单纯疱疹病毒(herpes simplex virus,HSV)-1感染的影响。方法将HSV-1注入小鼠前房建立动物模型。分别于建模前1d,建模后1d和4d结膜下注射TNF-α-ASON和PBS。8d和10d后观察两组小鼠眼部的炎症改变,测定病毒复制和眼内TNF-α含量。结果与对照组相比,8d时实验组葡萄膜炎发病率和眼内TNF-α含量显著降低(P<0.01),眼内HSV-1滴度无明显改变;10d时葡萄膜炎发病率和眼内HSV-1滴度都显著升高。结论局部使用TNF-α-ASON可以显著降低HSV-1感染小鼠眼内TNF-α含量,降低视网膜炎发病率。TNF-α在眼内具有抑制HSV-1复制的作用。Objective To investigate the effect of TNF-α antisense oligonucleotides treatment on subconjunctival herpes simplex virus（HSV）-1-induced uveitis.Methods HSV-1 was inoculated into the anterior chamber of mice.TNF-α-ASON and PBS were respectively injected subconjunctival into mice at-1 d,1 d and 4 d.The clinical course of retinitis,viral titer in the eyes and contents of TNF-α were analyzed.Results At day-8,TNF-α-ASON reduced the incidence of uveitis and the expression of TNF-α（P〈0.01）.The level of virus was not changed.However,the incidence of uveitis and viral titers were increased at day-10.Conclusions Topical TNF-α-ASON can reduce the contents of TNF-α and the incidence of uveitis.TNF-α may have an antiviral activity against HSV-1.

关 键 词：肿瘤坏死因子 反义寡核苷酸 单纯疱疹病毒 

分 类 号：R773.9[医药卫生—眼科]