脉冲场凝胶电泳在沙门氏菌流行病学中的应用  

Pulsed Field Gel Electrophoresis and Its Application in Epidemiology of Salmonella

作　　者：陈瑞[1,2] 熊惠军[2] 宋立[1] 陈坚[1,3] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]华南农业大学兽医学院,广州510642 [3]广东永顺生物制药有限公司,广州511356

出　　处：《中国兽药杂志》2010年第7期37-39,43,共4页Chinese Journal of Veterinary Drug

基　　金：国家"十一五"科技支撑子课题(2006BAK02A03-1)

摘　　要：脉冲场凝胶电泳是20世纪80年代发展起来的一种可用于分离10 kb至10 Mb分子质量DNA的新型凝胶电泳技术,已广泛应用于细菌分子分型、种群特异性鉴定及遗传分析等方面。文章介绍了脉冲场凝胶电泳的技术、应用状况及目前沙门氏菌的分型现状,并阐述了该技术在沙门氏菌流行病学研究方面的应用及发展前景。Pulsed Field Gel Electrophoresis（PFGE） is a new technique of gel electrophoresis developed in 1980＇s.It can be used to separate large DNA molecules ranging from 10 kb to 10 Mb.It has been widely applied to the bacterial molecular typing,identification of species groups and genetic analysis,etc.The technology and application of PFGE and the current status of molecular typing of salmonella were introduced in this article,and its application in epidemiological study of salmonella and development prospects were discussed.

关 键 词：脉冲场凝胶电泳 沙门氏菌 分子分型 流行病学 

分 类 号：S852.612[农业科学—基础兽医学]