溃疡性结肠炎多药耐药基因的表达及其与疾病行为间的关系  

Relationship between expression of multidrug-resistant genes in ulcerative colitis and disease behavior

作　　者：张英剑[1] 李建生[1] 金建军[2] 赵双琴[2] 

机构地区：[1]郑州大学第一附属医院消化科,郑州450052 [2]河南科技大学第一附属医院消化科

出　　处：《中华消化内镜杂志》2010年第7期360-362,共3页Chinese Journal of Digestive Endoscopy

摘　　要：目的 探讨溃疡性结肠炎多药耐药基因（MDR1）产物P-gp和多药耐药相关蛋白（MRP）的表达及其与疾病行为间的关系.方法 应用多药耐药基因（MDR1）产物P-gp和多药耐药相关蛋白（MRP）单克隆抗体,对溃疡性结肠炎患者内镜活检组织进行免疫组化检测.结果 多药耐药基因（MDR1）产物P-gp和多药耐药相关蛋白（MRP）在溃疡性结肠炎病程12个月、18个月时表达率分别为40.5%、45.9%、48.6%和51.4%,分别与患病初期相比,差异有统计学意义（P〈0.05）;P-gp和MRP在溃疡性结肠炎活动期和缓解期表达率分别为36.4%、18.6%和46.1%、25.4%,两期分别相比差异有统计学意义（P〈0.05）;P-gp和MRP活动期轻度、中度和重度之间差异无统计学意义（P〉0.05）.结论 通过内镜活检组织P-gp和MRP检测可了解溃疡性结肠炎耐药情况,为临床治疗提供依据,方法安全可靠、简便可行.P-gp和MRP表达在活动期严重程度间无差异,不宜作为严重程度是否耐药判断指标.Objective To study the relationship between expression of multidrug resistant 1 （ MDR1） gene encoded P-glycoprotein （ P-gp） and multidrug resistance relation protein （ MRP） in ulcerative colitis and the disease behavior. Methods Sections of endoscopic biopsy samples from patients with ulcerative colitis were studied by immunohistochemistry with monoclonal antibody of MDR1 gene encoded P-gp and MRP. Results Expressions of MDR1 gene encoded P-gp and MRP in the 12th month and 18th month of ulcerative colitis were 40. 5% , 45.9% , 48. 6% and 51.4% , respectively, which were all significantly higher than those of early phase （ P 〈 0.05）. P-gp and MRP expressions in active stage and remission stage of ulcerative colitis were 36.4% , 18. 6% , 46. 1% and 25.4% , respectively, which was significantly different between the two stages （P 〈 0.05 ）. At active stage of ulcerative colitis there was no significant difference in expressions of P-gp and MRP among mild, moderate and severe patients （P 〉 0. 05 ）. Conclusion Examination of P-gp and MRP expressions is feasible and dependable in presenting evidence of drug insistence. However, it is not suitable to be an indicator of severity because of its poor differentiation ability in active stage.

关 键 词：结肠炎 溃疡性 基因表达 抗药性 多药 基因 MDR 

分 类 号：R574.62[医药卫生—消化系统]