草莓炭疽病生防菌株MT-06的鉴定及生物学特性  

Identification and characterization of the fungal strain MT-06 for biocontrol of strawberry anthracnose

作　　者：魏彩燕[1,2] 毛雪琴[2] 柴荣耀[2] 王艳丽[2] 王教瑜[2] 张震[2] 邱海萍[2] 姜华[2] 杜新法[2] 孙国昌[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004 [2]浙江省农业科学院植物保护与微生物研究所,杭州310021

出　　处：《菌物学报》2010年第4期481-487,共7页Mycosystema

摘　　要：对草莓炭疽病具有良好生防效果的菌株MT-06的抑菌谱、生物学特性及其分类地位进行了研究。结果表明:MT-06具有广谱抗菌性,对多种病原菌具有拮抗作用;其营养生长的最适条件是:在PDA或PSA培养基上,28-35℃,初始pH为5,24h全光照;在查氏培养基上,25-28℃,24h全光照条件下孢子产量最高。经形态观察和rDNA-ITS序列及β-tubulin序列分析,将该菌株鉴定为Talaromyces flavus(Klcker)Stolk & Samson。A study was conducted to determine the biological characteristics of the fungal strain MT-06 isolated from the strawberry tissue suffered from anthracnose disease,which was identified as Talaromyces flavus（Klcker） Stolk Samson based on morphologic observation and both rDNA-ITS and β-tubulin sequence.The results indicate that the colony of MT-06 grows better on the PDA and potato saccharose agar（PSA） media,while Czapek medium was most favorable to its conidial production.The optimal temperature for its colony growth and sporulation ranged from 28 to 35℃ and from 25 to 28℃,respectively.The optimal initial pH of culture media was pH5.The strain could grow in either dark or light,but lighting was beneficial for vegetative growth and sporulation in comparison with dark.The test of antimicrobial spectrum shows that MT-06 is able to inhibit many kinds of plant fungal pathogens in vitro.

关 键 词：TALAROMYCES FLAVUS 生物防治 rDNA-ITS及β-tubulin序列分析 拮抗作用 抑菌谱 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]