高效液相色谱法测定茶叶中的咖啡因  

Determination of Caffeine in Tea by HPLC

作　　者：薛萍[1] 

机构地区：[1]广东轻工职业技术学院轻化系,广东广州510300

出　　处：《安徽农业科学》2010年第17期8942-8943,共2页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金资助项目(06023571);广东省科技计划项目(2007B03010300)

摘　　要：[目的]建立HPLC法快速测定茶叶中的咖啡因。[方法]色谱柱Zorbax-C8（4.6 mm×250.0 mm,5.0μm）;流动相为0.04 mol/L柠檬酸水溶液∶N,N-二甲基甲酰胺（DMF）∶四氢呋喃（THF）=90∶16∶4;流速为1.00 ml/min;柱温60℃;检测波长280 nm;进样量为10μl。[结果]咖啡因在20~100μg/ml时,浓度与峰面积呈良好的线性关系（r=0.999 9）,加标回收率为98.9%~102.3%,相对标准偏差RSD为0.23%。[结论]该方法简单、快速、准确,适用于茶叶和茶饮料中咖啡因含量的测定。[Objective] The aim was to establish a rapid method by HPLC for determination of caffeine concentration in tea.[Method] The conditions were： chromatographic column Zorbax-C8（4.6 mm×250.0 mm,5.0 μm）,mobile phase consisting of a mixture of citric acid solution（0.04 mol/L）∶N,N-dimethyl formamide∶tetrahydrofuran=90∶16∶4,flow rate 1.00 ml/min,column temperature 60 ℃,detection wavelength 280 nm,the sample dose 10 μl.[Result] When the concentration of caffeine was 20-100 μg/ ml,the perfect linearity between concentration and peak area ratio was obtained（r=0.999 9）.The recovery rate was 98.9%-102.3%,the relative standard deviation was 0.23%.[Conclusion] The method is simple,rapid and accurate,and can be used for determination of caffeine concentration in tea and tea drinks.

关 键 词：HPLC 测定 茶叶 咖啡因 

分 类 号：S571.1[农业科学—茶叶生产加工]