反相高效液相色谱法测定浓缩苹果汁中的10种酚类物质  

DETERMINATION OF 10 POLYPHENOLS IN APPLE JUICE CONCENTRATED BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：孔祥虹[1,2] 何强 郝少莉 

机构地区：[1]陕西师范大学,西安710062 [2]陕西出入境检验检疫局,西安710068 [3]陕西省农业工程勘察设计院,西安710068

出　　处：《化学分析计量》2010年第4期19-21,共3页Chemical Analysis And Meterage

基　　金：国家质检总局科研项目(2007IK166);陕西省科技计划资助项目(2008K01-06)

摘　　要：建立反相高效液相色谱检测浓缩苹果汁中的多酚物质如二羟基苯甲酸、绿原酸、咖啡酸、对香豆酸、邻香豆酸、阿魏酸、槲皮素、异槲皮素等方法。HPLC条件为：色谱柱Insertsil ODS-3（250mm×4．6mm，5um），流动相为乙酸水溶液和乙腈，柱温35℃，流速0．8mL／min，线性梯度洗脱，DAD检测器波长为280nm。该方法的检出限为0．0012—0．025mg／L，10种酚类物质的加标回收率均大于85％，测定结果的相对标准偏差为0．9％～2．0％（n=5）。A method of reversed - phase high performance liquid chromatography for determination of polyphenols in apple juice concentrated such as 3,4-dihydroxybenzoic acid, chlorogenic acid, caffeic acid, P- coumaric acid, O- comnaric acid, ferullic acid, quecetin dihydrate and isoquecitin was established. The HPLC conditions were as follows： column insertsil ODS -3 （250 mm × 4.6 mm, 5 um） , mobile phase acetic acid - acetonitrile, column temperature 35℃, flow rate 0.8 mL/min, DAD detector with 280 nm wavelength. The detection limit of the method ranged from 0.001 2 to 0.025 mg/L, the recoveries of polyphenols were more than 85% , and the relative standard deviations were 0.9% - 2.0% （n = 5）.

关 键 词：反相高效液相色谱 浓缩苹果汁 多酚 

分 类 号：O657.72[理学—分析化学]