反相高效液相色谱法测定食品添加剂乳铁蛋白  

RP-HPLC Determination of Lactoferrin as Food Additive

作　　者：张林田[1] 陈小雪[1] 魏建华[1] 张冬辉[1] 陈建伟[1] 

机构地区：[1]汕头出入境检验检疫局,汕头515041

出　　处：《理化检验（化学分册）》2010年第7期765-766,771,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家质检总局项目(20071903-T-424)

摘　　要：建立了反相高效液相色谱法测定食品添加剂乳铁蛋白方法。样品采用0．5mol·^L-1氯化钠溶液溶解，稀释至适当浓度，过滤后供液相色谱仪测定，采用CAPCELL SG300 C18色谱柱分离。用两种不同配比的0．5mol·^L-1氯化钠溶液、乙腈及三氟乙酸的混合液作为流动相，梯度淋洗，在280nm波长处检测，保留时间其特征紫外吸收光谱为乳铁蛋白定性的依据，外标法峰面积作定量测定。乳铁蛋白的质量浓度在10．0-100．0mol·^L-1范围内与其对应的峰面积呈线性，测定下限（10S／N）为5．0mg·kg^-1。RP-HPLC was applied to the determination of lactoferrin, which is used as a food additive. Sample of lactoferrin was dissolved with 0. 5 mol·^L-1 NaCl solution and diluted to an appropriate concentration with the same solvent. After filtration on 0. 45 um filtration membrane, the solution was used for HPLC analysis. The CAPCELL SG300 C18 chromatographic column was used for separation. Mixtures containing acetonitrile, 0. 5 mol·^L-1 NaCl solution and trifluoroacetic acid mixed in different ratio were used as the mobile phase in gradient elution. UV-detection at the wavelength of 280 nm with DAD was adopted in the detection. Retention time and its characteristic UV-absorption spectra were the basis for qualification of lactoferrin. External standard method was used for the determination of lactoferrin. Linear relationship between values of peak area and mass concentration of lactoferrin was obtained in the range of 10. 0--100. 0 mg·L^-1. Its lower limit of determination （10S/N） was found to be 5.0mg·kg^-1.

关 键 词：反相高效液相色谱法 添加剂 乳铁蛋白 

分 类 号：O652.63[理学—分析化学]