儿童B系急性淋巴细胞白血病TEL-AML1融合基因的研究  

Study of TEL AML1 fusion gene in childhood B lineage acute lymphoblastic leukemia

作　　者：牛超[1,2,3] 黄薇[1,2,3] 苏欣莹[1,2,3] 曹琪[1,2,3] 顾柏炜[1,2,3] 贾培敏[1,2,3] 顾龙君[1,2,3] 蒋慧[1,2,3] 陈竺[1,2,3] 陈赛娟[1,2,3] 

机构地区：[1]上海第二医科大学附属瑞金医院上海血液学研究所 [2]上海第二医科大学附属新华医院 [3]上海儿童医院

出　　处：《中华血液学杂志》1999年第2期61-64,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金;上海血液学研究所胡应洲基金

摘　　要：目的了解ＴＥＬＡＭＬ１融合基因在儿童Ｂ系急性淋巴细胞白血病（急淋）中的发生率及其与临床诊断和预后的关系，并比较巢式逆转录聚合酶链反应（ＲＴＰＣＲ）和双标记荧光原位杂交（ＦＩＳＨ）两种方法。方法采用巢式ＲＴＰＣＲ和双标记ＦＩＳＨ技术检测ＴＥＬＡＭＬ１融合基因。结果和结论ＲＴＰＣＲ检测发现儿童Ｂ系急淋中２２．５％（４０例中９例）的患者有ＴＥＬＡＭＬ１融合基因转录本，这类患者预后较好，完全缓解率达１００％。ＲＴＰＣＲ和ＦＩＳＨ技术是检测ＴＥＬＡＭＬ１的有效方法，并为临床诊断和预后估计提供了有一定价值的手段。Objective To determine the incidence of TEL AML1 fusion gene and its role in clinical diagnosis and prognosis of childhood B lineage acute lymphoblastic leukemia (B ALL), and to compare the two techniques: nested RT PCR and dual color fluorescence in situ hybridization (FISH). Methods Nested RT PCR and dual color FISH techniques were used to detect TEL AML1 fusion gene. Results and Conclusion TEL AML1 fusion transcript was found in 22.5%(9/40) of patients with B ALL by RT PCR. The prognosis of these TEL AML1 positive patients was relatively good and the complete remission rate was 100%. Both RT PCR and FISH techniques were proved to be useful for detecting TEL AML1 fusion gene and suitable for clinical diagnosis and prognosis evaluation.

关 键 词：白血病 ALL 聚合酶链反应 TEL-AML1 融合基因 

分 类 号：R733.710.4[医药卫生—肿瘤]