添加胰蛋白酶促进Streptomyces hygroscopicus CCTCC M203062 产谷氨酰胺转胺酶？  

Enhancement of Microbial Transglutaminase by Streptomyces hygroscopicus CCTCC M203062 Using Tripsin

作　　者：程力[1,2] 张东旭 堵国成 陈坚[1,3] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,无锡214122 [2]江南大学食品学院,无锡214122 [3]江南大学工业乍物技术教育部重点实验室,无锡214122

出　　处：《中国生物工程杂志》2010年第7期53-58,共6页China Biotechnology

基　　金：国家自然科学基金(30770055,20776063);江苏省高新技术研究项目(BG2007009);江苏省自然科学基金(BK2007018)资助项目

摘　　要：研究了添加胰蛋白酶对Streptomyces hygroscopicus CCTCC M203062合成谷氨酰胺转胺酶的影响。结果表明,添加胰蛋白酶可以提高发酵过程中谷氨酰胺转胺酶的酶活。摇瓶培养中,在发酵起始时添加200U/ml的胰蛋白酶,谷氨酰胺转胺酶的酶活最高达到了6.61U/ml,比对照提高了27.1%。初步研究表明,添加胰蛋白酶可以直接切割发酵过程中产生的酶原,使其被快速地转化为成熟酶,因此推测胰蛋白酶提高谷氨酰胺转胺酶酶活的原因是解除了酶原的产物抑制作用,产生更多的酶原,从而促进了产酶。Effects of Tripsin addition on the accumulation of microbial transglutaminase（MTG） by Streptomyces hygroscopicus CCTCC M203062 were investigated. The results showed that the addition of Trypsin enhanced MTG accumulation, and the optimal addition time and concentration of Trypsin were at 0 h and 200 U/ml. The maximum MTG activity in the culture broth was 6.61 U/ml and increased by 27.1% compared with the control. With the addition of Trypsin, pro-TGase was cut to MTG, thus we presume adding Trypsin into the culture broth can enhance the production of pro-TGase by relieving the product inhibition, and the accumulation of MTG was improved.

关 键 词：谷氨酰胺转胺酶 STREPTOMYCES HYGROSCOPICUS 胰蛋白酶 酶原 

分 类 号：Q933[生物学—微生物学]