CENP-E变异体在不同肿瘤细胞株及癌组织中的表达差异  

Difference in Expressions of CENP-E Variant in Various Tumor Cell Strains and Carcinoma Tissues

作　　者：闫琛[1] 凌康[2] 刘子杰[1] 翁亚光[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学教育部重点实验室重庆市重点实验室,重庆400016 [2]重庆医科大学第一临床学院外科,重庆400016

出　　处：《中国生物制品学杂志》2010年第7期678-682,691,共6页Chinese Journal of Biologicals

基　　金：教育部博士点基金资助项目(20060631012)

摘　　要：目的研究CENP-E变异体(CENP-EⅠ)在不同肿瘤细胞株及癌组织和相应的癌旁组织中的表达情况。方法应用巢式RT-PCR检测14个细胞株和17份癌组织及相应癌旁组织标本中CENP-EⅠmRNA(缺失第38个外显子)的表达情况,并比较变异体和野生型CENP-E(CENP-EWT) mRNA的表达差异;应用巢式PCR检测上述标本中CENP-E在DNA水平上的变异;并通过细胞增殖试验、细胞周期检测和染色体核型鉴定,分析CENP-EⅠ与细胞恶性程度的相关性。结果 RT-PCR结果显示,实验样本中CENP-E mRNA均有CENP-EWT和CENP-EⅠ两种形式同时存在于一种组织或细胞中,且在正常细胞株和癌旁组织中CENP-EWT表达量较高,在肿瘤细胞株和癌组织中CENP-EⅠ表达量较高,且差异有统计学意义(P<0.05);样本中CENP-E在DNA水平上不存在第38个外显子缺失,且细胞间CENP-E表达量的差异无统计学意义(P>0.05);CENP-EⅠ与细胞的恶性程度有一定的相关性。结论同一细胞内存在2种或2种以上形式的CENP-E mRNA,CENP-E变异体可能与肿瘤的发生有关。Objective To investigate the expressions of CENP-EⅠ,a CENP-E variant,in various tumor cell strains as well as peri-cancerous and carcinoma tissues.Methods The expressions of mRNA of CENP-EⅠ,a CENP-E variant with deletion of the 38th exon,in 14 cell strains as well as 17 carcinoma tissue specimens and their corresponding peri-cancerous tissue specimens were determined by nested RT-PCR,and compared with those of wild type CENP-E（CENP-EWT）.The variations of CENP-E at DNA level in the above-mentioned specimens were determined by nested PCR.The relationship of CENP-EⅠ to the malignant degree of cells were analyzed by cell proliferation test,determination of cell cycle and identification of chromosome karyotype.Results RT-PCR proved that both CENP-EWT and CENP-EⅠexisted in the same tissue or cell strain.CENP-EWT was highly expressed in normal cell strains and peri-cancerous tissues,while CENP-EⅠ in tumor cell strains and carcinoma tissues,which showed significant difference（P 0.05）.No deletion of the 38th exon at DNA level was observed in the specimens,and the expression levels of CENP-E in various cells showed no significant difference.However,CENP-EⅠ showed a certain relationship to the malignant degree of cells.Conclusion The CENP-E mRNA in not less than two forms may exist in the same cell strain,and CENP-E variant may be related to tumorigenesis.

关 键 词：纺锤体检查点 CENP-E 变异体 肿瘤 

分 类 号：R730.231[医药卫生—肿瘤]