高效溴氨酸脱色菌的分离鉴定及其特性研究  

Investigation on the isolation and characterization of high-efficient bromamine acid-decolorizing strains

作　　者：周元祥[1] 石倩倩[1] 叶红曼[1] 

机构地区：[1]合肥工业大学资源与环境工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2010年第7期1057-1061,共5页Journal of Hefei University of Technology：Natural Science

基　　金：国家高技术研究863发展计划资助项目(2007AA021303)

摘　　要：文章从ABR反应器污泥中筛选到1株对溴氨酸具有较好脱色作用的细菌E2,通过对这株细菌的形态观察、生理生化特征鉴定及16S rDNA序列测定,认为菌株E2是Enterobacter ludwigii。菌株E2对溴氨酸脱色的最佳碳、氮源分别为葡萄糖和硫酸铵;最适碳源质量浓度为1 g/L,质量浓度过高,脱色效果反而不显著;最适氮源质量浓度为0.5 g/L,质量浓度增大,脱色率基本不变;磷酸盐对脱色无显著影响;菌株E2对溴氨酸脱色的最佳pH值为7-8,最适温度为30-35℃,最适接种量为5%;随溴氨酸质量浓度的增加,脱色率降低,但对于400 mg/L的溴氨酸,脱色率仍可达80.5%;在所考察盐度范围内,盐度对脱色无显著影响。溴氨酸经生物脱色降解后产生一种新产物,此产物的最大吸收波长为410 nm。A strain E2 that had a capability to decolorize bromamine acid（BAA）was isolated from the sludge samples of anaerobic baffled reactor（ABR）.In the light of its morphological and physiological features as well as its 16S rDNA sequences,it was identified as Enterobacter ludwigii.The most preferable carbon sources and nitrogen sources for decolorization of bromamine acid are glucose and（NH4）2SO4 respectively.The optimal concentration of carbon sources is 1 g/L,and when the concentration of glucose is more than 2 g/L,the decolorization effect is not good.Besides,the optimal concentration of nitrogen sources is 0.5 g/L,and when the concentration increases,the decolorization rate remains basically unchanged.Phosphate has little effect on the decolorization.The optimal growth conditions are pH of 7-8,temperature of 30-35 ℃ and inoculation amount of 5 %.When the concentration of bromamine acid increases,the decolorization rate decreases,but decolorization rate at concentration of 400 mg/L is still up to 80.5 %.In a range of 0-25 g/L,salinity has little effect on the decolorization.The bromanine acid is degraded to form a new product with maximum absorption wavelength at 410 nm.

关 键 词：溴氨酸 脱色菌 菌株筛选 

分 类 号：X172[环境科学与工程—环境科学] X703.1