成纤维细胞生长因子受体1(FGFR-1)基因在人肠癌组织中的变化  

CHANGES OF FGFR 1 GENOMIC DNA IN HUMAN COLORECTAL CARCINOMA

作　　者：常雅萍[1,2] 苗传龙[1,2] 冯乐平 娄刚毅[1,2] 于永利 

机构地区：[1]白求恩医科大学免疫学教研室 [2]吉林省肿瘤防治研究所

出　　处：《免疫学杂志》1999年第2期90-92,共3页Immunological Journal

基　　金：国家跨世纪优秀人才基金及香港求是基金

摘　　要：为观察人ＦＧＦＲ－１基因变化与结肠癌发生的关系。采用ＲＴ－ＰＣＲ法从人肺成纤维细胞中钓取ＦＧＦＲ－１全编码区ｃＤＮＡ，用此ｃＤＮＡ探针与人肠癌及癌旁组织ＤＮＡ进行Ｓｏｕｔｈｅｒｎ印迹分析。结果表明：与癌旁组织相比结肠癌组织Ｓｏｕｔｈｅｒｎ印迹图谱发生了改变。提示ＰＧＦＲ－１基因变化与肠癌发生可能存在一定相关性。To detect the relevance between the changes of FGFR 1 genomic DNA and the generation of colo rectal carcinoma,the cDNA encoding FGFR 1 region was isolated by RT PCR from human fibroblasts, then used as a probe to analyze genomic DNAs of colonal carcinoma and adjacent tissues in Southern blotting. The results showed that the Southern blotting maps of colonal carcinama were different from those of adjacent tissues. The variance of FGFR 1 genomic DNA may have certain connection with the development of colonal carcinoma.

关 键 词：FGFR-1 结肠癌 癌组织 SOUTHERN杂交 基因表达 

分 类 号：R735.350.2[医药卫生—肿瘤] Q78[医药卫生—临床医学]