茶多酚在体外诱导HL-60细胞的凋亡  

Induction of apoptosis by tea polyphenols in HL-60 cells

作　　者：赵燕[1] 曹进[1] 刘箭卫[1] 祝和成[1] 马洪 

机构地区：[1]湖南医科大学茶与健康研究室

出　　处：《中国病理生理杂志》1999年第3期264-267,共4页Chinese Journal of Pathophysiology

摘　　要：目的：寻找新的肿瘤细胞凋亡诱导剂。方法：选用茶的主要活性成分茶多酚，采用噻唑蓝（ＭＴＴ）还原法，ＤＮＡ凝胶电泳以及透射电镜技术，在体外观察了茶多酚对人急性早幼粒白血病细胞（ＨＬ－６０）凋亡的影响。结果：被２５０ｍｇ／Ｌ茶多酚作用５ｈ后ＨＬ－６０细胞进行凝胶电泳呈现出典型的ＤＮＡ条带，透射电镜下看到凋亡小体，茶多酚对ＨＬ－６０细胞凋亡的诱导活性平行于它的细胞杀伤作用。结论：在体外培养条件下。AIM To study anticarcinogenic mechanism of polyphenolic compounds in tea.METHODS: The apoptosis inducing effects of tea polyphenols (TP), the main active component purified from tea, were observed on the human promyelocytic leukemic cell (HL-60) by thiazolyl blue tetrazolium bromide reduction test, DNA agarose gel electrophoresis and transmission electronic microscopy technique.RESULTS:After treating HL-60 cells with TP in 250mg·L 1 for 5h, DNA extracted from HL-60 cells showed a typical internucleosomal DNA degradation, i.e., DNA ladder and apoptosis vehicles were observed. The effect of TP was showed to parallel with its cytotoxic activity in HL-60 cells.CONCLUSION:The mechanism of antitumor action of TP is related to its apoptosis inducing activity.

关 键 词：白血病 HL-60细胞 细胞凋亡 茶多酚 

分 类 号：R733.7[医药卫生—肿瘤]