黄曲霉毒素产毒菌株的快速筛选法  

A Rapid Screening Method of Aflatoxicogenic Strains

作　　者：李云[1] 周应揆[1] 曹玉 李劲松 

机构地区：[1]云南大学生物系

出　　处：《云南大学学报（自然科学版）》1999年第2期162-164,共3页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省科委国际科技合作项目

摘　　要：运用花生察氏培养基、花生葡萄糖硝酸铵培养基、筛选培养基，这三种培养基对从谷物、食品、饲料等环境中分离出的１２株黄曲霉菌株的产毒能力进行了筛选试验．根据黄曲霉毒素在紫外灯下能发出强烈荧光的特点进行试验，筛选出４株黄曲霉毒素产毒菌株．经过菌株产毒培养、毒素的抽提、薄层层析等一系列试验证明，这三种荧光筛选培养基能够对黄曲霉毒素产毒菌株进行快速有效的筛选．Applied three kinds of media (PCD,PGAN,SM) to screen aflatoxicogenic strains from 12 strains of Aspergillus flavus isolated from grain,food,feed and so on.According to the intense fluorescence of aflatoxin under UV light,we screened out 4 aflatoxicogenic strains.The three kinds of fluorescence screening media can rapidly and effectively screen out aflatoxicogenic strains,which was proved by a series of experiments including aflatoxin producing culture,aflatoxin extracts and thin layer chromatography.

关 键 词：黄曲霉毒素 荧光筛选法 筛选法 黄曲霉菌 

分 类 号：Q949.327.1[生物学—植物学] R996.2[医药卫生—毒理学]