表面活性剂沉淀鱼精蛋白及复溶回收过程研究  

Surfactant Precipitation and Recovery of Protamine

作　　者：刘琳[1,2] 付朝霞[3] 刘俊果[1,2] 杜立业[1] 赵紫华[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018 [2]河北省发酵工程技术研究中心,河北石家庄050018 [3]河北科技大学科研处,河北石家庄050018

出　　处：《食品科学》2010年第13期56-59,共4页Food Science

基　　金：河北省教育科学研究计划项目(2006315);河北科技大学学科建设项目(2006JC-13)

摘　　要：采用两种阴离子型表面活性剂:十二烷基硫酸钠(SDS)和2-乙基己基琥珀酸酯磺酸钠(AOT)沉淀鱼精蛋白并用极性有机溶剂从沉淀中复溶回收鱼精蛋白。结果表明:SDS可以完全沉淀鱼精蛋白,而表面活性剂AOT最高只能沉淀75.02%的鱼精蛋白;极性有机溶剂的种类及辅助无机盐的添加量,对从鱼精蛋白-表面活性剂难溶复合物中回收鱼精蛋白有很大的影响;对于SDS-鱼精蛋白复合物,正丙醇为最佳回收剂,鱼精蛋白最高回收率可达83.33%;对于AOT-鱼精蛋白复合物,丙酮为最佳回收剂,鱼精蛋白最高回收率达到85.71%。回收后的鱼精蛋白抗菌活性同原始鱼精蛋白相比没有明显变化。The precipitation and recovery of protamine was investigated using ionic surfactants as precipitating ligands and polar organic solvents as recovery agents. Ionic surfactant such as sodium dodecyl sulfate （SDS） exhibited a complete removal of protamine; however, sodium di-（2-ethylhexyl） sulfosuccinate （AOT） exhibited only 75.02% removal of protamine. In addition, an obvious effect of organic solvent and salt amount on the recovery of protamine from its precipitate. As for SDS-protamine precipitate, n-propanol was the optimal solvent with protamine recovery rate of more than 83.33%. As for AOT-protamine precipitate, acetone was the optimal solvent with protamine recovery rate of more than 85.71%. Compared with original protamine, no significant change in antibacterial activity of recovered protamine was observed.

关 键 词：鱼精蛋白 表面活性剂 沉淀 纯化 

分 类 号：TQ932[化学工程]