杜仲绿原酸的分离纯化及结构鉴定  

Purification and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves

作　　者：卢琪[1] 段家彩[1] 高丽[1] 陈若雯[1] 傅虹飞[1] 潘思轶[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2010年第14期275-279,共5页Food Science

摘　　要：筛选大孔树脂纯化澄清后的杜仲绿原酸粗提液,并对纯化后的绿原酸进行初步的结构鉴定。采用壳聚糖、活性炭澄清绿原酸的粗提液,选取7种不同类型的大孔树脂对杜仲绿原酸进行静态吸附解吸实验比较。结果表明,NKA-2型大孔树脂对绿原酸有较好的选择性,其吸附率和解吸率分别为99.67%和62.8%。盐酸调整洗脱液的pH值为3,洗脱液的体积分数为30%时,洗脱效果最好。澄清后的绿原酸粗提液经乙酸乙酯萃取除去黄酮,再用NKA-2型树脂进行梯度洗脱,可得到纯度为76.3%的绿原酸。HPLC-MS分析表明,绿原酸是咖啡酸和奎尼酸缩合的缩酚酸。Eucommia ulmoides leaves were extracted with 60% aqueous ethanol under the assistance of ultrasound.The crude extract was then condensed on a rotation evaporator,added with chitosan acetic acid solution and stirred,allowed to stand at 60 ℃ for 60 min for precipitate sedimentation,and filtrated.The filtrate was decolorized with activated carbon prior to 5-time ethyl acetate extraction to remove flavonoids.The lower phase was collected and a chlorpgenic acid extract was obtained.Seven types of moroporous resins were used to purify the chlorpgenic acid extract,and NKA-2 was the selected resin because it exhibited good selectivity to chlorpgenic acid and the static adsorption and desorption ratios were 99.67% and 62.8%.The use of 30% acidified ethanol （pH 3） provided an optimum elution.After NKA-2 purification in the gradient elution mode,a chlorpgenic acid product with 76.3% purity was obtained.Chlorpgenic acid was confirmed by HPLC-MS analysis to be a condensation product of caffeic acid and quinic acid.

关 键 词：绿原酸 大孔树脂 纯化 鉴定 

分 类 号：Q946.81[生物学—植物学] R931.71[医药卫生—生药学]