载脂肪酶壳聚糖/海藻酸钙微胶囊的制备  

Preparation of alginate/chitosan microcapsule with lipase immobilization

作　　者：刘博[1] 张宏亮[2] 薛伟明[1] 齐智涛[3] 田志敏[3] 太琴花[1] 

机构地区：[1]西北大学化工学院,陕西西安710069 [2]中国石化集团洛阳石油化工工程公司,河南洛阳471003 [3]西北大学生命科学学院,陕西西安710069

出　　处：《化工进展》2010年第8期1541-1546,共6页Chemical Industry and Engineering Progress

基　　金：陕西省"13115"科技创新工程重大科技专项(2008ZDKG-58);陕西省教育厅科技计划项目(07JK386);西北大学研究生自主创新资助项目(08YZZ49;07YZZ23)

摘　　要：针对固定化脂肪酶的研究背景,以壳聚糖、海藻酸钠为微载体制备材料,采用脉冲电场液滴工艺制备壳聚糖/海藻酸钙微胶囊。以脂肪酶为生物模型,系统考察了制备条件对载脂肪酶壳聚糖/海藻酸钙微胶囊酶活力的影响。结果表明:海藻酸钠质量浓度和酶与海藻酸钠载体配比是影响固定化酶活力的主要因素,载酶量为15mg/mL,海藻酸钠质量浓度为10mg/mL时载酶微胶囊酶活力最高,球形度好。通过改变壳聚糖质量浓度和相对分子质量,可以调控微胶囊膜的厚密程度进而影响固定化酶活力。成膜液pH值依次影响壳聚糖与海藻酸盐分子中官能团的电离状态、成膜反应静电络合程度、酶蛋白包封率,最终影响固定化酶活力。在载酶量为15mg/mL,海藻酸钠质量浓度为10mg/mL,壳聚糖相对分子质量、质量浓度和pH值依次为50kDa、1mg/mL和3.0的条件下,固定化酶活力为187IU/g。Using chitosan and sodium alginate as micro-carrier material,calcium-alginate-chitosan microcapsules were prepared by an impulsive electrostatic preparation process. Enzyme activity of lipase immobilized in these microcapsules under different preparation conditions was investigated. Results indicated that the enzyme activity were mainly affected by lipase/alginate ratio and alginate mass concentration. The highest enzyme activity was found with 15 mg/mL enzyme loading and regular spherical microcapsules were obtained with 10 mg/mL alginate mass concentration. The microcapsule membrane creation was affected by both the relative molar mass and mass concentration of chitosan,through which the enzyme activity was also influenced. The pH value of chitosan solution regulated the ionization state of functional groups in both chitosan and alginate molecules,and hence the electrostatic binding in membrane generation,the enzyme protein encapsulation rate,and finally the enzyme activity. A maximum enzyme activity of 187 IU/g was achieved ultimately under preparation conditions with 15 mg/mL of lipase/alginate ratio,10 mg/mL of alginate mass concentration,50kDa of chitosan relative molar mass and 1 mg/mL of chitosan mass concentration with pH 3.0.

关 键 词：脂肪酶 固定化 壳聚糖 海藻酸钠 微胶囊 

分 类 号：Q814.2[生物学—生物工程]